The role of K+ influx on glutamate induced astrocyte swelling: effect of temperature.

A. S. Bender, Michael D Norenberg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied factors involved in glutamate-stimulated astrocyte swelling and the role of temperature in this process. Glutamate-induced astrocyte swelling was reduced by 74% when temperature was lowered from 37 degrees C to 24 degrees C. Reduction in temperature to 24 degrees C also resulted in inhibition of glutamate-stimulated K+ uptake. When both extracellular and intracellular Ca2+ were removed with BAP-TA/AM (50 microM) and 0.1 mM EGTA and in the absence of extracellular Ca2+, glutamate was not able to stimulate the K+ uptake or swelling. Dantrolene (20 microM), MK-801 (0.5 mM) and ouabain (0.5 mM) inhibited glutamate-induced K+ uptake as well as the glutamate effects on swelling. These findings show the importance of Ca2+ in the mechanism of glutamate swelling and suggests that glutamate induces swelling through stimulation of K+ influx and that the diminished effect of glutamate swelling with decrease in temperature may be due to a decrease in K+ uptake.

Original languageEnglish
Pages (from-to)28-30
Number of pages3
JournalActa Neurochirurgica, Supplement
Volume60
StatePublished - Jan 1 1994

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glutamates
Astrocytes
swelling
Swelling
Glutamic Acid
Temperature
temperature
Dantrolene
Dizocilpine Maleate
Egtazic Acid
Ouabain
stimulation

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology

Cite this

The role of K+ influx on glutamate induced astrocyte swelling : effect of temperature. / Bender, A. S.; Norenberg, Michael D.

In: Acta Neurochirurgica, Supplement, Vol. 60, 01.01.1994, p. 28-30.

Research output: Contribution to journalArticle

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