The role of intracellular pH changes in heat sensitization by procaine

Eric Wieder, M. H. Fox

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although it has been known for many years that procaine sensitizes cells markedly to hyperthermia, the mechanism by which this occurs is not yet understood. The recent finding in our laboratory that procaine caused an intracellular acidification following heating prompted further studies using carboxy-SNARF-1 to measure the intracellular pH of cells during heating. We found that procaine actually causes an intracellular alkalinization during heating and the intracellular pH is lowered immediately after the extracellular procaine is removed. These results suggest that procaine causes acid loading analogous to ammonium chloride (NH4Cl) loading. Sensitization could not be attributed entirely to this acid shock, since a comparable acid shock with NH4Cl loading following heating caused only a slight sensitization compared to procaine. Heated cells acidified with NH4Cl loading recovered rapidly from the low intracellular pH, whereas the cells acidified with procaine loading did not. Cell sorting demonstrated that the cells which were unable to recover from acidification by procaine had significantly lower survival than those that recovered. Thus, in addition to causing an intracellular acidification following heating, procaine alters cellular processes responsible for recovery from an acid shock.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalRadiation Research
Volume133
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

procaine
Procaine
Hot Temperature
heat
heating
Heating
cells
acids
shock
acidification
Shock
Acids
ammonium chlorides
hyperthermia
causes
classifying
recovery
Ammonium Chloride
alkalinization
ammonium chloride

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

The role of intracellular pH changes in heat sensitization by procaine. / Wieder, Eric; Fox, M. H.

In: Radiation Research, Vol. 133, No. 1, 1993, p. 67-72.

Research output: Contribution to journalArticle

@article{708c4e04d5a1495484edf29e36c78363,
title = "The role of intracellular pH changes in heat sensitization by procaine",
abstract = "Although it has been known for many years that procaine sensitizes cells markedly to hyperthermia, the mechanism by which this occurs is not yet understood. The recent finding in our laboratory that procaine caused an intracellular acidification following heating prompted further studies using carboxy-SNARF-1 to measure the intracellular pH of cells during heating. We found that procaine actually causes an intracellular alkalinization during heating and the intracellular pH is lowered immediately after the extracellular procaine is removed. These results suggest that procaine causes acid loading analogous to ammonium chloride (NH4Cl) loading. Sensitization could not be attributed entirely to this acid shock, since a comparable acid shock with NH4Cl loading following heating caused only a slight sensitization compared to procaine. Heated cells acidified with NH4Cl loading recovered rapidly from the low intracellular pH, whereas the cells acidified with procaine loading did not. Cell sorting demonstrated that the cells which were unable to recover from acidification by procaine had significantly lower survival than those that recovered. Thus, in addition to causing an intracellular acidification following heating, procaine alters cellular processes responsible for recovery from an acid shock.",
author = "Eric Wieder and Fox, {M. H.}",
year = "1993",
language = "English (US)",
volume = "133",
pages = "67--72",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "1",

}

TY - JOUR

T1 - The role of intracellular pH changes in heat sensitization by procaine

AU - Wieder, Eric

AU - Fox, M. H.

PY - 1993

Y1 - 1993

N2 - Although it has been known for many years that procaine sensitizes cells markedly to hyperthermia, the mechanism by which this occurs is not yet understood. The recent finding in our laboratory that procaine caused an intracellular acidification following heating prompted further studies using carboxy-SNARF-1 to measure the intracellular pH of cells during heating. We found that procaine actually causes an intracellular alkalinization during heating and the intracellular pH is lowered immediately after the extracellular procaine is removed. These results suggest that procaine causes acid loading analogous to ammonium chloride (NH4Cl) loading. Sensitization could not be attributed entirely to this acid shock, since a comparable acid shock with NH4Cl loading following heating caused only a slight sensitization compared to procaine. Heated cells acidified with NH4Cl loading recovered rapidly from the low intracellular pH, whereas the cells acidified with procaine loading did not. Cell sorting demonstrated that the cells which were unable to recover from acidification by procaine had significantly lower survival than those that recovered. Thus, in addition to causing an intracellular acidification following heating, procaine alters cellular processes responsible for recovery from an acid shock.

AB - Although it has been known for many years that procaine sensitizes cells markedly to hyperthermia, the mechanism by which this occurs is not yet understood. The recent finding in our laboratory that procaine caused an intracellular acidification following heating prompted further studies using carboxy-SNARF-1 to measure the intracellular pH of cells during heating. We found that procaine actually causes an intracellular alkalinization during heating and the intracellular pH is lowered immediately after the extracellular procaine is removed. These results suggest that procaine causes acid loading analogous to ammonium chloride (NH4Cl) loading. Sensitization could not be attributed entirely to this acid shock, since a comparable acid shock with NH4Cl loading following heating caused only a slight sensitization compared to procaine. Heated cells acidified with NH4Cl loading recovered rapidly from the low intracellular pH, whereas the cells acidified with procaine loading did not. Cell sorting demonstrated that the cells which were unable to recover from acidification by procaine had significantly lower survival than those that recovered. Thus, in addition to causing an intracellular acidification following heating, procaine alters cellular processes responsible for recovery from an acid shock.

UR - http://www.scopus.com/inward/record.url?scp=0027457381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027457381&partnerID=8YFLogxK

M3 - Article

VL - 133

SP - 67

EP - 72

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 1

ER -