Abstract
Inositides have an important impact on diverse areas of cellular regulation. However, since this area has grown exponentially from the mid 1980s onwards, many workers find themselves relatively new to the field. In this chapter, we establish a broad foundation for the rest of the book by covering some important principles of inositide methodologies. The focus is especially directed to those methods or aspects of methodology not covered in detail in other chapters. This includes the often neglected influence of the inositide precursor, inositol, and important background information relating to the labelling and extraction of inositides from cells and tissues. This introductory section also gives a "birds eye" view of important methods and protocols found within this volume and hopefully acts as a touchstone to assess which of the methodologies described within this book is most appropriate for your particular study(ies) of inositides.
| Original language | English |
|---|---|
| Pages (from-to) | 1-19 |
| Number of pages | 19 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 645 |
| DOIs | |
| State | Published - Nov 8 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
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The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells. / Barker, Christopher J.; Berggren, Per Olof.
In: Methods in molecular biology (Clifton, N.J.), Vol. 645, 08.11.2010, p. 1-19.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells.
AU - Barker, Christopher J.
AU - Berggren, Per Olof
PY - 2010/11/8
Y1 - 2010/11/8
N2 - Inositides have an important impact on diverse areas of cellular regulation. However, since this area has grown exponentially from the mid 1980s onwards, many workers find themselves relatively new to the field. In this chapter, we establish a broad foundation for the rest of the book by covering some important principles of inositide methodologies. The focus is especially directed to those methods or aspects of methodology not covered in detail in other chapters. This includes the often neglected influence of the inositide precursor, inositol, and important background information relating to the labelling and extraction of inositides from cells and tissues. This introductory section also gives a "birds eye" view of important methods and protocols found within this volume and hopefully acts as a touchstone to assess which of the methodologies described within this book is most appropriate for your particular study(ies) of inositides.
AB - Inositides have an important impact on diverse areas of cellular regulation. However, since this area has grown exponentially from the mid 1980s onwards, many workers find themselves relatively new to the field. In this chapter, we establish a broad foundation for the rest of the book by covering some important principles of inositide methodologies. The focus is especially directed to those methods or aspects of methodology not covered in detail in other chapters. This includes the often neglected influence of the inositide precursor, inositol, and important background information relating to the labelling and extraction of inositides from cells and tissues. This introductory section also gives a "birds eye" view of important methods and protocols found within this volume and hopefully acts as a touchstone to assess which of the methodologies described within this book is most appropriate for your particular study(ies) of inositides.
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UR - http://www.scopus.com/inward/citedby.url?scp=78049411285&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-175-2_1
DO - 10.1007/978-1-60327-175-2_1
M3 - Article
C2 - 20645178
AN - SCOPUS:78049411285
VL - 645
SP - 1
EP - 19
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -