O papel do eixo intestino-fígado na restrição de crescimento intra-uterino em um modelo de gastrosquise experimental

Translated title of the contribution: The role of gut-liver axis in the restriction of intrauterine growth in a model of experimental gastroschisis

Márcia Pereira Bueno, Frances Lilian Lanhellas Gonçalves, Dioze Guadagnini, Ricardo Barini, Mario José Abdalla Saad, Rodrigo Melo Gallindo, Augusto Frederico Schmidt, Lourenço Sbragia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

PURPOSE: To evaluate the intrauterine growth restriction (IUGR) by the expression of IR-β, IRS-1, IRS-2, IGF-IRβ and Ikappaβ in experimental model of gastroschisis. METHODS: Pregnant rats at 18.5 days of gestation were submitted to surgery to create experimental fetal gastroschisis (term = 22 days) were divided in three groups: gastroschisis (G), control (C) and sham (S). Fetuses were evaluated for body weight (BW), intestinal (IW), liver (LW) and their relations IW/BW and LW/BW. IR-β and IGF-IRβ receptors, IRS-1 and IRS-2 substrates and Ikappaβ protein were analyzed by western blotting. RESULTS: BW was lower in G, the IW and IW / BW were greater than C and S (p<0.05) groups. The liver showed no differences between groups. In fetuses with gastroschisis, compared with control fetuses, the expression of IGF-IRβ (p<0.001) and Ikappaβ (p<0.001) increased in the liver and intestine, as well as IR-β (p<0.001) which decreased in both. In contrast to the intestine, IRS-1 (p<0.001) increased in the liver and IRS-2 decreased (p<0.01). CONCLUSION: The axis of the intestine liver has an important role in inflammation, with consequent changes in the metabolic pathway of glucose can contribute to the IUGR in fetuses with gastroschisis.

Translated title of the contributionThe role of gut-liver axis in the restriction of intrauterine growth in a model of experimental gastroschisis
Original languagePortuguese
Pages (from-to)3-7
Number of pages5
JournalActa Cirurgica Brasileira
Volume28
Issue numberSUPPL.1
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • Fetal development
  • Fetal growth retardation
  • Gastroschisis
  • Insulin
  • Rats
  • Receptor

ASJC Scopus subject areas

  • Surgery

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