Abstract
L1 is a neural cell adhesion molecule critical for neural development. Full-length L1 (L1FL) contains an alternatively spliced cytoplasmic sequence, RSLE, which is absent in L1 expressed in nonneuronal cells. The RSLE sequence follows a tyrosine, creating an endocytic motif that allows rapid internalization via clathrin-mediated endocytosis. We hypothesized that L1FL would internalize more rapidly than L1 lacking the RSLE sequence (L1ΔRSLE) and that internalization might regulate L1- mediated adhesion. L1 internalization was measured by immunofluorescence microscopy and by uptake of 125I-anti-rat-L1 antibody, demonstrating that L1FL is internalized 2-3 times faster than L1ΔRSLE. Inhibition of clathrin-mediated endocytosis slowed internalization of L1FL but did not affect initial uptake of L1ΔRSLE. To test whether L1 endocytosis regulates L1 adhesion, cell aggregation rates were tested. L1ΔRSLE cells aggregated two times faster than L1FL cells. Inhibition of clathrin-mediated endocytosis increases the aggregation rate of the L1FL cells to that of L1ΔRSLE cells. Our results demonstrate that rapid internalization of L1 dramatically affects L1 adhesion.
| Original language | English |
|---|---|
| Pages (from-to) | 1285-1290 |
| Number of pages | 6 |
| Journal | Journal of Biological Chemistry |
| Volume | 276 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 12 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
The role of endocytosis in regulating L1-mediated adhesion. / Long, Kristin E.; Asou, Hiroaki; Snider, Martin D.; Lemmon, Vance.
In: Journal of Biological Chemistry, Vol. 276, No. 2, 12.01.2001, p. 1285-1290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of endocytosis in regulating L1-mediated adhesion
AU - Long, Kristin E.
AU - Asou, Hiroaki
AU - Snider, Martin D.
AU - Lemmon, Vance
PY - 2001/1/12
Y1 - 2001/1/12
N2 - L1 is a neural cell adhesion molecule critical for neural development. Full-length L1 (L1FL) contains an alternatively spliced cytoplasmic sequence, RSLE, which is absent in L1 expressed in nonneuronal cells. The RSLE sequence follows a tyrosine, creating an endocytic motif that allows rapid internalization via clathrin-mediated endocytosis. We hypothesized that L1FL would internalize more rapidly than L1 lacking the RSLE sequence (L1ΔRSLE) and that internalization might regulate L1- mediated adhesion. L1 internalization was measured by immunofluorescence microscopy and by uptake of 125I-anti-rat-L1 antibody, demonstrating that L1FL is internalized 2-3 times faster than L1ΔRSLE. Inhibition of clathrin-mediated endocytosis slowed internalization of L1FL but did not affect initial uptake of L1ΔRSLE. To test whether L1 endocytosis regulates L1 adhesion, cell aggregation rates were tested. L1ΔRSLE cells aggregated two times faster than L1FL cells. Inhibition of clathrin-mediated endocytosis increases the aggregation rate of the L1FL cells to that of L1ΔRSLE cells. Our results demonstrate that rapid internalization of L1 dramatically affects L1 adhesion.
AB - L1 is a neural cell adhesion molecule critical for neural development. Full-length L1 (L1FL) contains an alternatively spliced cytoplasmic sequence, RSLE, which is absent in L1 expressed in nonneuronal cells. The RSLE sequence follows a tyrosine, creating an endocytic motif that allows rapid internalization via clathrin-mediated endocytosis. We hypothesized that L1FL would internalize more rapidly than L1 lacking the RSLE sequence (L1ΔRSLE) and that internalization might regulate L1- mediated adhesion. L1 internalization was measured by immunofluorescence microscopy and by uptake of 125I-anti-rat-L1 antibody, demonstrating that L1FL is internalized 2-3 times faster than L1ΔRSLE. Inhibition of clathrin-mediated endocytosis slowed internalization of L1FL but did not affect initial uptake of L1ΔRSLE. To test whether L1 endocytosis regulates L1 adhesion, cell aggregation rates were tested. L1ΔRSLE cells aggregated two times faster than L1FL cells. Inhibition of clathrin-mediated endocytosis increases the aggregation rate of the L1FL cells to that of L1ΔRSLE cells. Our results demonstrate that rapid internalization of L1 dramatically affects L1 adhesion.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035846950&partnerID=8YFLogxK
U2 - 10.1074/jbc.M006658200
DO - 10.1074/jbc.M006658200
M3 - Article
C2 - 11035015
AN - SCOPUS:0035846950
VL - 276
SP - 1285
EP - 1290
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 2
ER -