The role of depletion of dimethyl sulfoxide before autografting: On hematologic recovery, side effects, and toxicity

R. Syme, M. Bewick, D. Stewart, K. Porter, T. Chadderton, Stefan Glück

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Cryopreservation of stem cells after collection from peripheral blood or bone marrow for autologous transplantation necessitates protection with dimethyl sulfoxide (DMSO). Unfortunately, DMSO, when infused with the thawed cell suspension, may induce serious complications and side effects. To assess whether depletion of DMSO before autografting affects safety and efficacy, 56 consenting consecutive patients treated with high-dose chemotherapy and autologous blood stem cell transplantation were assigned to obtain either an untreated or DMSO-depleted autograft. On the day of transplantation, the cryopreserved cells were thawed and infused to the patient either immediately or after washing 3 times in normal saline supplemented with 6% anticoagulant citrate dextrose solution. Cell count with viability, clonogenic assay, and phenotyping were performed before and after thawing and after washing. Hematologic recovery, side effects, and complications were recorded. The in vitro and clinical data on 56 patients show that the depletion of DMSO in vitro before autografting does not induce a significant loss of cell number, viability, colony-forming unit-granulocyte-macrophage activity, or number of CD34+ cells. Furthermore, it leads to a safe and sustained engraftment. The complications and side effects, as recorded by continuous monitoring, were substantially less; however, the procedure takes 3 to 4 hours of laboratory work per patient.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalBiology of Blood and Marrow Transplantation
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Autologous Transplantation
Dimethyl Sulfoxide
Cell Count
Granulocyte-Macrophage Progenitor Cells
Cryopreservation
Cell Transplantation
Autografts
Stem Cell Transplantation
Bone Marrow Transplantation
Citric Acid
Anticoagulants
Blood Cells
Cell Survival
Suspensions
Stem Cells
Safety
Glucose
Drug Therapy
In Vitro Techniques

Keywords

  • Autologous blood stem cell transplantation
  • Cryopreservation
  • Dimethyl sulfoxide
  • High-dose chemotherapy

ASJC Scopus subject areas

  • Transplantation

Cite this

The role of depletion of dimethyl sulfoxide before autografting : On hematologic recovery, side effects, and toxicity. / Syme, R.; Bewick, M.; Stewart, D.; Porter, K.; Chadderton, T.; Glück, Stefan.

In: Biology of Blood and Marrow Transplantation, Vol. 10, No. 2, 01.02.2004, p. 135-141.

Research output: Contribution to journalArticle

Syme, R. ; Bewick, M. ; Stewart, D. ; Porter, K. ; Chadderton, T. ; Glück, Stefan. / The role of depletion of dimethyl sulfoxide before autografting : On hematologic recovery, side effects, and toxicity. In: Biology of Blood and Marrow Transplantation. 2004 ; Vol. 10, No. 2. pp. 135-141.
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