The role of apoptosis in the early corneal wound healing after excimer laser keratectomy in the rat

Qian Li, M. Farooq Ashraf, Nicoletta A. Bekoe, Walter J. Stark, Chi Chao Chan, Terrence O'Brien

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: The potential role of apoptosis in corneal wound healing after excimer laser keratectomy was investigated in a rat model. Methods: Lewis rats underwent laser keratectomy using a 193-nm excimer laser. The central corneas were ablated in three depths: group A, epithelium; group B, superficial stroma; group C, deep stroma. Eyes were collected at 1, 12, 24, and 36 h and 1 week. Cellular markers associated with apoptosis - Fas, Fas ligand (FasL), Bcl-2, and Bax - were examined by immunohisto-chemistry. Keratocyte depletion and endothelial changes were evaluated histologically. In situ end labeling of double-stranded DNA breaks was used to demonstrate apoptosis in corneal sections. Results: Keratocyte depletion was observed in 6 (50%) of 12 rats (total from groups A, B, and C) at 12 h, 11 (73%) of 15 at 24 h, 3 (20%) of 15 at 36 h, and 2 (15%) of 13 at 1 week after laser surgery. Corneal endothelial edema was observed in the ablation zone. Expression of Fas, FasL, Bcl-2, and Bax in corneal cells showed dynamics similar to that of keratocyte depletion and endothelial changes. There was less expression of apoptotic molecules in newly generated epithelial cells and more in endothelial cells of the stromal ablation groups. Conclusions: Excimer laser keratectomy triggered apoptosis of corneal keratocytes and endothelial cells. More endothelial edema was observed in the stromal ablation than in the epithelial ablation group. The expression of apoptotic molecules coincided with the period of keratocyte depletion and regeneration and of endothelial recovery, suggesting that apoptosis is a dynamic part of corneal wound healing and remodeling after excimer laser keratectomy.

Original languageEnglish
Pages (from-to)853-860
Number of pages8
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume238
Issue number10
DOIs
StatePublished - Nov 13 2000
Externally publishedYes

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Laser Corneal Surgery
Excimer Lasers
Wound Healing
Apoptosis
Fas Ligand Protein
Corneal Keratocytes
Endothelial Cells
Corneal Edema
Double-Stranded DNA Breaks
Laser Therapy
Cornea
Regeneration
Edema
Epithelium
Epithelial Cells

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The role of apoptosis in the early corneal wound healing after excimer laser keratectomy in the rat. / Li, Qian; Ashraf, M. Farooq; Bekoe, Nicoletta A.; Stark, Walter J.; Chan, Chi Chao; O'Brien, Terrence.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 238, No. 10, 13.11.2000, p. 853-860.

Research output: Contribution to journalArticle

Li, Qian ; Ashraf, M. Farooq ; Bekoe, Nicoletta A. ; Stark, Walter J. ; Chan, Chi Chao ; O'Brien, Terrence. / The role of apoptosis in the early corneal wound healing after excimer laser keratectomy in the rat. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2000 ; Vol. 238, No. 10. pp. 853-860.
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abstract = "Background: The potential role of apoptosis in corneal wound healing after excimer laser keratectomy was investigated in a rat model. Methods: Lewis rats underwent laser keratectomy using a 193-nm excimer laser. The central corneas were ablated in three depths: group A, epithelium; group B, superficial stroma; group C, deep stroma. Eyes were collected at 1, 12, 24, and 36 h and 1 week. Cellular markers associated with apoptosis - Fas, Fas ligand (FasL), Bcl-2, and Bax - were examined by immunohisto-chemistry. Keratocyte depletion and endothelial changes were evaluated histologically. In situ end labeling of double-stranded DNA breaks was used to demonstrate apoptosis in corneal sections. Results: Keratocyte depletion was observed in 6 (50{\%}) of 12 rats (total from groups A, B, and C) at 12 h, 11 (73{\%}) of 15 at 24 h, 3 (20{\%}) of 15 at 36 h, and 2 (15{\%}) of 13 at 1 week after laser surgery. Corneal endothelial edema was observed in the ablation zone. Expression of Fas, FasL, Bcl-2, and Bax in corneal cells showed dynamics similar to that of keratocyte depletion and endothelial changes. There was less expression of apoptotic molecules in newly generated epithelial cells and more in endothelial cells of the stromal ablation groups. Conclusions: Excimer laser keratectomy triggered apoptosis of corneal keratocytes and endothelial cells. More endothelial edema was observed in the stromal ablation than in the epithelial ablation group. The expression of apoptotic molecules coincided with the period of keratocyte depletion and regeneration and of endothelial recovery, suggesting that apoptosis is a dynamic part of corneal wound healing and remodeling after excimer laser keratectomy.",
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AU - Chan, Chi Chao

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N2 - Background: The potential role of apoptosis in corneal wound healing after excimer laser keratectomy was investigated in a rat model. Methods: Lewis rats underwent laser keratectomy using a 193-nm excimer laser. The central corneas were ablated in three depths: group A, epithelium; group B, superficial stroma; group C, deep stroma. Eyes were collected at 1, 12, 24, and 36 h and 1 week. Cellular markers associated with apoptosis - Fas, Fas ligand (FasL), Bcl-2, and Bax - were examined by immunohisto-chemistry. Keratocyte depletion and endothelial changes were evaluated histologically. In situ end labeling of double-stranded DNA breaks was used to demonstrate apoptosis in corneal sections. Results: Keratocyte depletion was observed in 6 (50%) of 12 rats (total from groups A, B, and C) at 12 h, 11 (73%) of 15 at 24 h, 3 (20%) of 15 at 36 h, and 2 (15%) of 13 at 1 week after laser surgery. Corneal endothelial edema was observed in the ablation zone. Expression of Fas, FasL, Bcl-2, and Bax in corneal cells showed dynamics similar to that of keratocyte depletion and endothelial changes. There was less expression of apoptotic molecules in newly generated epithelial cells and more in endothelial cells of the stromal ablation groups. Conclusions: Excimer laser keratectomy triggered apoptosis of corneal keratocytes and endothelial cells. More endothelial edema was observed in the stromal ablation than in the epithelial ablation group. The expression of apoptotic molecules coincided with the period of keratocyte depletion and regeneration and of endothelial recovery, suggesting that apoptosis is a dynamic part of corneal wound healing and remodeling after excimer laser keratectomy.

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