The role of an adherent cell in the production of monocyte chemotactic factor

James E. Vogel; Savita G. Pahwa; Elizabeth M. Smithwick; Robert A. Good

doi:10.1016/0165-2478(81)90004-3

The role of an adherent cell in the production of monocyte chemotactic factor

James E. Vogel, Savita G. Pahwa, Elizabeth M. Smithwick, Robert A. Good

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The functional role of an adherent cell in assisting a variety of in vitro immune responses is well established. An assay for human monocyte chemotaxis in vitro was utilized as a means of investigating the role of adherent cells in the production of the monocyte chemotactic factor that is produced by peripheral blood lymphocytes upon stimulation with Concanavalin A. Depletion of a population of adherent cells by passage of peripheral blood mononuclear cells through a Sephadex G-10 column rendered the latter incapable of producing monocyte chemotactic factor. The requirement for adherent cells in the production of a 'lymphokine' is in agreement with many previous works of a similar nature performed in other experimental systems.

Original language	English (US)
Pages (from-to)	273-276
Number of pages	4
Journal	Immunology Letters
Volume	3
Issue number	5
DOIs	https://doi.org/10.1016/0165-2478(81)90004-3
State	Published - Nov 1981
Externally published	Yes

Keywords

adherent cells
chemotaxis
lymphokines
Sephadex G-10 column filtration

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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10.1016/0165-2478(81)90004-3

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title = "The role of an adherent cell in the production of monocyte chemotactic factor",

abstract = "The functional role of an adherent cell in assisting a variety of in vitro immune responses is well established. An assay for human monocyte chemotaxis in vitro was utilized as a means of investigating the role of adherent cells in the production of the monocyte chemotactic factor that is produced by peripheral blood lymphocytes upon stimulation with Concanavalin A. Depletion of a population of adherent cells by passage of peripheral blood mononuclear cells through a Sephadex G-10 column rendered the latter incapable of producing monocyte chemotactic factor. The requirement for adherent cells in the production of a 'lymphokine' is in agreement with many previous works of a similar nature performed in other experimental systems.",

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year = "1981",

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T1 - The role of an adherent cell in the production of monocyte chemotactic factor

AU - Vogel, James E.

AU - Pahwa, Savita G.

AU - Smithwick, Elizabeth M.

AU - Good, Robert A.

PY - 1981/11

Y1 - 1981/11

N2 - The functional role of an adherent cell in assisting a variety of in vitro immune responses is well established. An assay for human monocyte chemotaxis in vitro was utilized as a means of investigating the role of adherent cells in the production of the monocyte chemotactic factor that is produced by peripheral blood lymphocytes upon stimulation with Concanavalin A. Depletion of a population of adherent cells by passage of peripheral blood mononuclear cells through a Sephadex G-10 column rendered the latter incapable of producing monocyte chemotactic factor. The requirement for adherent cells in the production of a 'lymphokine' is in agreement with many previous works of a similar nature performed in other experimental systems.

AB - The functional role of an adherent cell in assisting a variety of in vitro immune responses is well established. An assay for human monocyte chemotaxis in vitro was utilized as a means of investigating the role of adherent cells in the production of the monocyte chemotactic factor that is produced by peripheral blood lymphocytes upon stimulation with Concanavalin A. Depletion of a population of adherent cells by passage of peripheral blood mononuclear cells through a Sephadex G-10 column rendered the latter incapable of producing monocyte chemotactic factor. The requirement for adherent cells in the production of a 'lymphokine' is in agreement with many previous works of a similar nature performed in other experimental systems.

KW - adherent cells

KW - chemotaxis

KW - lymphokines

KW - Sephadex G-10 column filtration

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JO - Immunology Letters

JF - Immunology Letters

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ER -

The role of an adherent cell in the production of monocyte chemotactic factor

Abstract

Keywords

ASJC Scopus subject areas

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