Abstract
The functional role of an adherent cell in assisting a variety of in vitro immune responses is well established. An assay for human monocyte chemotaxis in vitro was utilized as a means of investigating the role of adherent cells in the production of the monocyte chemotactic factor that is produced by peripheral blood lymphocytes upon stimulation with Concanavalin A. Depletion of a population of adherent cells by passage of peripheral blood mononuclear cells through a Sephadex G-10 column rendered the latter incapable of producing monocyte chemotactic factor. The requirement for adherent cells in the production of a 'lymphokine' is in agreement with many previous works of a similar nature performed in other experimental systems.
Original language | English (US) |
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Pages (from-to) | 273-276 |
Number of pages | 4 |
Journal | Immunology Letters |
Volume | 3 |
Issue number | 5 |
DOIs | |
State | Published - Nov 1981 |
Externally published | Yes |
Keywords
- adherent cells
- chemotaxis
- lymphokines
- Sephadex G-10 column filtration
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy