The RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN

Benjamin Ezraty, Brian Dahlgren, Murray P Deutscher

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In eukaryotes, archaea, and in some eubacteria, removal of 3′ precursor sequences during maturation of tRNA is catalyzed by an endoribonuclease, termed RNase Z. In contrast, in Escherichia, coli, a variety of exoribonucleases carry out final 3′ maturation. Yet, E. coli retains an. RNase Z homologue, ElaC, whose function is under active study. We have overexpressed and purified to homogeneity His-tagged ElaC and show here that it is, in fact, the previously described enzyme, RNase BN. Thus, purified ElaC displays structural and catalytic properties identical to those ascribed to RNase BN. In addition, an elaC mutant strain behaves identically to a known RNase BN- strain, CAN. Finally, we show that wild type elaC can complement the mutation, in strain CAN and that the elaC gene in strain CAN carries a nonsense mutation that results in loss of RNase BN activity. These data correct a previous misassignment for the gene encoding RNase BN. Based on the fact that the original MNase BN imitation has now been identified, we propose that the elaC gene be renamed rbn.

Original languageEnglish
Pages (from-to)16542-16545
Number of pages4
JournalJournal of Biological Chemistry
Volume280
Issue number17
DOIs
StatePublished - Apr 29 2005

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Escherichia coli
Genes
Exoribonucleases
Endoribonucleases
Gene encoding
Nonsense Codon
Archaea
Transfer RNA
Eukaryota
RNase Z
ribonuclease BN
Bacteria
Mutation
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

The RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN. / Ezraty, Benjamin; Dahlgren, Brian; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 280, No. 17, 29.04.2005, p. 16542-16545.

Research output: Contribution to journalArticle

Ezraty, Benjamin ; Dahlgren, Brian ; Deutscher, Murray P. / The RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 17. pp. 16542-16545.
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