The repeated sequence CATT(A/T) is required for granulocyte-macrophage colony-stimulating factor promoter activity

Stephen D Nimer, J. Fraser, J. Richards, M. Lynch, J. Gasson

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The hematopoietic growth factor GM-CSF (granulocyte-macrophage colony-stimulating factor) is expressed by activated but not resting T lymphocytes. Previously, we localized GM-CSF-inducible promoter activity to a 90-bp region of GM-CSF 5′-flanking sequences extending from bp -53 to +37. To more precisely identify the GM-CSF DNA sequences required for inducible promoter activity in T lymphocytes, we have performed mutagenesis within a region of GM-CSF 5′-flanking sequences (bp -57 to -24) that contains the repeated sequence CATT(A/T). Mutations that do not alter the repeated CATT(A/T) sequence do not eliminate inducible promoter activity, whereas mutation or deletion of either of the CATT(A/T) repeats eliminates all inducible promoter activity in T-cell lines and in primary human T lymphocytes. Thus, both copies of the direct repeat CATT(A/T) are required for mitogen-inducible expression of GM-CSF in T cells.

Original languageEnglish
Pages (from-to)6084-6088
Number of pages5
JournalMolecular and Cellular Biology
Volume10
Issue number11
StatePublished - Dec 1 1990
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor
T-Lymphocytes
5' Flanking Region
Sequence Deletion
Nucleic Acid Repetitive Sequences
Mitogens
Mutagenesis
Intercellular Signaling Peptides and Proteins
Cell Line
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The repeated sequence CATT(A/T) is required for granulocyte-macrophage colony-stimulating factor promoter activity. / Nimer, Stephen D; Fraser, J.; Richards, J.; Lynch, M.; Gasson, J.

In: Molecular and Cellular Biology, Vol. 10, No. 11, 01.12.1990, p. 6084-6088.

Research output: Contribution to journalArticle

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AB - The hematopoietic growth factor GM-CSF (granulocyte-macrophage colony-stimulating factor) is expressed by activated but not resting T lymphocytes. Previously, we localized GM-CSF-inducible promoter activity to a 90-bp region of GM-CSF 5′-flanking sequences extending from bp -53 to +37. To more precisely identify the GM-CSF DNA sequences required for inducible promoter activity in T lymphocytes, we have performed mutagenesis within a region of GM-CSF 5′-flanking sequences (bp -57 to -24) that contains the repeated sequence CATT(A/T). Mutations that do not alter the repeated CATT(A/T) sequence do not eliminate inducible promoter activity, whereas mutation or deletion of either of the CATT(A/T) repeats eliminates all inducible promoter activity in T-cell lines and in primary human T lymphocytes. Thus, both copies of the direct repeat CATT(A/T) are required for mitogen-inducible expression of GM-CSF in T cells.

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