The relationship between vaccinia virus DNA replication and intermediate filaments

Wendou Yu, Q. Yu, M. X. Ding, Z. H. Zhai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Various DNA components which were extracted with gentle cell fractionation from the HeLa cells after 4 h vaccinia virus infection were detected by dot hybridization technique. The virus DNA mainly exist in intermediate filament-lamina-nuclear matrix complex. With DGD embedment free technique and electron microscopic autoradiography, the newly synthesized virus DNA is found to be associated with intermediate filaments. The results of southwestern hybridization demonstrate that vaccinia virus DNA has specific affinity to intermediate filaments and some nuclear matrix proteins.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalShi yan sheng wu xue bao = Journal of experimental biology
Volume26
Issue number3
StatePublished - Sep 1993
Externally publishedYes

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Intermediate Filaments
Vaccinia virus
Virus Replication
DNA Replication
DNA Viruses
Nuclear Matrix-Associated Proteins
Cell Fractionation
Nuclear Matrix
DNA
Virus Diseases
Autoradiography
HeLa Cells
Electrons

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The relationship between vaccinia virus DNA replication and intermediate filaments. / Yu, Wendou; Yu, Q.; Ding, M. X.; Zhai, Z. H.

In: Shi yan sheng wu xue bao = Journal of experimental biology, Vol. 26, No. 3, 09.1993, p. 239-247.

Research output: Contribution to journalArticle

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