The regulation of progesterone receptor by 17β estradiol and tamoxifen in the ZR-75-1 human breast cancer cell line in defined medium

Joseph C. Allegra, Orly Korat, Hoan M.T. Do, Marc Lippman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Abstract The regulation of progesterone receptor by 17β estradiol and tamoxifen in the ZR-75-1 human breast cancer cell line in defined medium is described. ZR-75-1 cells maintained in serum free hormone supplemented medium minus estradiol lack progesterone receptor activity. Readdition of estradiol to these cells leads to a marked stimulation of progesterone receptor activity (0 to greater than 100 fmols of specifically bound progesterone per million cells). Tamoxifen (10-6M-10-8M) does not stimulate progesterone receptor activity in this cell line. The presence of progesterone receptor activity is not directly related to growth. Withdrawal of insulin in the continued presence of estradiol has no effect on progesterone receptor concentration although net cell growth ceases. Conversely, withdrawal of estradiol in the continued presence of insulin induces a cessation of net cell growth accompanied by a loss of all progesterone receptor activity within 3-5 days.

Original languageEnglish (US)
Pages (from-to)17-27
Number of pages11
JournalJournal of Receptors and Signal Transduction
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The regulation of progesterone receptor by 17β estradiol and tamoxifen in the ZR-75-1 human breast cancer cell line in defined medium'. Together they form a unique fingerprint.

  • Cite this