During development of the nervous system receptor tyrosine kinases and receptor protein tyrosine phosphatases act in a coordinate way during axon growth and guidance. In the developing avian retinotectal system, many different receptor protein tyrosine phosphatases are expressed. Most of them have unknown functions. Retinal ganglion cells express at least three different members of this receptor family on their axons and growth cones: CRYPα, CRYP-2 and PTPμ. CRYPα interacts heterophilically with at least two different ligands found in the basal membranes of the retina and the optic tectum. To analyze the role of the CRYPα-ligand interaction, retinal ganglion cell axons were grown on retinal basal membranes (inner limiting membrane) and the receptor-ligand interaction was blocked from both the receptor side (by receptor specific antibodies) and from the ligand side by using a receptor-alkaline phosphatase fusion protein. Both of these treatments reduced average retinal axon length and induced a dramatic change in morphology of retinal ganglion cell growth cones on basal membranes, but not on other substrates like laminin, N-cadherin, matrigel- and detergent, treated basal membranes. These results suggest that CRYPα and its ligand act as growth-promoting molecules during intraretinal axon growth. (C) 2000 John Wiley and Sons, Inc.
|Original language||English (US)|
|Number of pages||15|
|Journal||Journal of Neurobiology|
|State||Published - Aug 24 2000|
- Axon guidance
- Retinotectal system
ASJC Scopus subject areas