The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells

D. K. Simoneaux, F. A. Fletcher, Roland Jurecic, H. G. Shilling, N. T. Van, P. Patel, J. W. Belmont

Research output: Contribution to journalArticle

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Abstract

We attempted to isolate novel receptor tyrosine kinase, which may play a role in hematopoietic development by screening for expressed sequences with conserved tyrosine kinase catalytic domains. Among the known tyrosine kinases identified in this screen, we found a gene with characteristics of a receptor tyrosine kinase but unusual motifs in the catalytic domain. This gene is identical to ryk described independently by other investigators. Chromosomal fluorescence in situ hybridization localization of human ryk was clarified by using monochromosomal hybrids and placing it as a single locus in 3q22. Although Northern analysis reveals widespread expression in adult mouse tissues, we have found that ryk expression is not ubiquitous. Expression increased in bone marrow cells from mice treated with 5-fluorouracil. Northern analysis on cell lines indicates expression in CD3-, CD4-, CD8- T cells (at a low level), pre-T cells, thymic epithelial cells, and mature myeloid cells, but not myeloid precursors or B cell precursors. Expression analysis with the use of RT-PCR on mouse bone marrow cells separated on the basis of cell surface markers (B220, CD4, CD8, Gr-1, Mac-1) reveals that this receptor is expressed in differentiated cells (Lin+) but is not expressed in the precursor cells (Lin-). Flow cytometric analysis with a monospecific anti-Ryk Ab demonstrates that Ryk+ cells constitute 36.7% and Lin+/Ryk+ cells constitute 33.7% of low density bone marrow cells whereas Ryk+ cells represent only 0.3% of the Lin- population. We conclude that ryk expression is regulated during hematopoietic development by lineage commitment and stage of maturation.

Original languageEnglish
Pages (from-to)1157-1166
Number of pages10
JournalJournal of Immunology
Volume154
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

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Receptor Protein-Tyrosine Kinases
Bone Marrow Cells
Genes
Myeloid Cells
Protein-Tyrosine Kinases
Catalytic Domain
Macrophage-1 Antigen
T-Lymphocytes
B-Lymphoid Precursor Cells
Conserved Sequence
Fluorescence In Situ Hybridization
Fluorouracil
Epithelial Cells
Research Personnel
Cell Line
Polymerase Chain Reaction
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Simoneaux, D. K., Fletcher, F. A., Jurecic, R., Shilling, H. G., Van, N. T., Patel, P., & Belmont, J. W. (1995). The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells. Journal of Immunology, 154(3), 1157-1166.

The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells. / Simoneaux, D. K.; Fletcher, F. A.; Jurecic, Roland; Shilling, H. G.; Van, N. T.; Patel, P.; Belmont, J. W.

In: Journal of Immunology, Vol. 154, No. 3, 01.01.1995, p. 1157-1166.

Research output: Contribution to journalArticle

Simoneaux, DK, Fletcher, FA, Jurecic, R, Shilling, HG, Van, NT, Patel, P & Belmont, JW 1995, 'The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells', Journal of Immunology, vol. 154, no. 3, pp. 1157-1166.
Simoneaux, D. K. ; Fletcher, F. A. ; Jurecic, Roland ; Shilling, H. G. ; Van, N. T. ; Patel, P. ; Belmont, J. W. / The receptor tyrosine kinase-related gene (ryk) demonstrates lineage and stage-specific expression in hematopoietic cells. In: Journal of Immunology. 1995 ; Vol. 154, No. 3. pp. 1157-1166.
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