The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines

Brian Marples, D. Longhurst, A. M. Eastham, C. M.L. West

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The single-cell gel electrophoresis (comet) assay was used to measure radiation-produced DNA double-strand breaks (dsbs) in a series of seven cervical tumour cell lines (ME180, HT3, C33A, C41, SiHa, MS751 and CaSki). The proportion of DNA dsbs was measured immediately after radiation treatment (initial damage) and 16 h later after incubation at 37°C (residual damage). Linear dose-response curves were seen for initial (slopes 0.23-0.66) and residual (slopes 0.16-0.87) DNA dsbs. Neither of the slopes of the linear regression analysis on the initial and on the residual DNA dsbs dose-response curves (range 0-80 Gy) correlated with SF2 (surviving fraction at 2 Gy) measured after high- (HDR) or low-dose-rate (LDR) irradiation. An association was evident between SF2 after HDR and LDR irradiation and the ratio of the absolute level of initial and residual damage after a single dose of 60 Gy. However, a significant correlation was found between HDR (r = -0.78, P = 0.04) and LDR (r = -0.86, P = 0.03) SF2 values and the ratio of the slopes of the initial and residual DNA dsbs dose-response curves (range 0.47-0.99), representing the fraction of DNA damage remaining. These results indicate that the neutral comet assay can be used to predict radiosensitivity of cervical tumour cell lines by assessing the ratio of initial and residual DNA dsbs.

Original languageEnglish (US)
Pages (from-to)1108-1114
Number of pages7
JournalBritish Journal of Cancer
Volume77
Issue number7
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Double-Stranded DNA Breaks
Radiation Tolerance
Cervix Uteri
DNA Damage
Carcinoma
Cell Line
Comet Assay
Tumor Cell Line
Radiation
Linear Models
Regression Analysis

Keywords

  • Cervix cancer
  • Comet assay
  • Intrinsic radiosensitivity
  • Predictive assay

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines. / Marples, Brian; Longhurst, D.; Eastham, A. M.; West, C. M.L.

In: British Journal of Cancer, Vol. 77, No. 7, 01.01.1998, p. 1108-1114.

Research output: Contribution to journalArticle

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