Abstract
The rat model of endocarditis is a well established experimental protocol which closely approximates human native valve endocarditis. The rat model of endocarditis has been used to examine the role of particular streptococcal virulence factors, to assess immunoprotective strategies, and to evaluate the efficacy of selected antibiotic treatment regimens for streptococcal endocarditis. Like humans, rats are generally susceptible to endocarditis only if the cardiac valves have been damaged. In the rat model of endocarditis, damage to the aortic valve and sterile vegetation formation is accomplished by insertion of a polyethylene catheter through the carotid artery into the left ventricle. Following catheter insertion, an inoculum of streptococci are injected intravenously. Vegetations removed from the heart valves during thoracotomy of euthanized animals are qualitatively cultured for streptococcal infection. The method, including investigator safety considerations, is described in detail.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Methods in Cell Science |
| Volume | 20 |
| Issue number | 1-4 |
| DOIs | |
| State | Published - Dec 1 1998 |
| Externally published | Yes |
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Keywords
- Endocarditis
- Rat
- Streptococci
ASJC Scopus subject areas
- Cell Biology
Cite this
The rat model of endocarditis. / Munro, Cindy.
In: Methods in Cell Science, Vol. 20, No. 1-4, 01.12.1998, p. 203-207.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The rat model of endocarditis
AU - Munro, Cindy
PY - 1998/12/1
Y1 - 1998/12/1
N2 - The rat model of endocarditis is a well established experimental protocol which closely approximates human native valve endocarditis. The rat model of endocarditis has been used to examine the role of particular streptococcal virulence factors, to assess immunoprotective strategies, and to evaluate the efficacy of selected antibiotic treatment regimens for streptococcal endocarditis. Like humans, rats are generally susceptible to endocarditis only if the cardiac valves have been damaged. In the rat model of endocarditis, damage to the aortic valve and sterile vegetation formation is accomplished by insertion of a polyethylene catheter through the carotid artery into the left ventricle. Following catheter insertion, an inoculum of streptococci are injected intravenously. Vegetations removed from the heart valves during thoracotomy of euthanized animals are qualitatively cultured for streptococcal infection. The method, including investigator safety considerations, is described in detail.
AB - The rat model of endocarditis is a well established experimental protocol which closely approximates human native valve endocarditis. The rat model of endocarditis has been used to examine the role of particular streptococcal virulence factors, to assess immunoprotective strategies, and to evaluate the efficacy of selected antibiotic treatment regimens for streptococcal endocarditis. Like humans, rats are generally susceptible to endocarditis only if the cardiac valves have been damaged. In the rat model of endocarditis, damage to the aortic valve and sterile vegetation formation is accomplished by insertion of a polyethylene catheter through the carotid artery into the left ventricle. Following catheter insertion, an inoculum of streptococci are injected intravenously. Vegetations removed from the heart valves during thoracotomy of euthanized animals are qualitatively cultured for streptococcal infection. The method, including investigator safety considerations, is described in detail.
KW - Endocarditis
KW - Rat
KW - Streptococci
UR - http://www.scopus.com/inward/record.url?scp=0032458675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032458675&partnerID=8YFLogxK
U2 - 10.1023/A:1009719802803
DO - 10.1023/A:1009719802803
M3 - Article
AN - SCOPUS:0032458675
VL - 20
SP - 203
EP - 207
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 1-4
ER -