The Rac2 guanosine triphosphatase regulates B lymphocyte antigen receptor responses and chemotaxis and is required for establishment of B-1a and marginal zone B lymphocytes

Ben A. Croker, David M. Tarlinton, Leonie A. Cluse, Alana J. Tuxen, Amanda Light, Feng-Chun Yang, David A. Williams, Andrew W. Roberts

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

We have defined roles for the hemopoietic-specific Rho guanosine triphosphatase, Rac2, in B lymphocyte development and function through examination of rac2-/- mice. Rac2-deficient mice displayed peripheral blood B lymphocytosis and marked reductions in peritoneal cavity B-1a lymphocytes, marginal zone B lymphocytes, and IgM-secreting plasma cells as well as reduced concentrations of serum IgM and IgA. The rac2-/- B lymphocytes exhibited reduced calcium flux following coligation of B cell AgR and CD19 and reduced chemotaxis in chemokine gradients. T cell-independent responses to DNP-dextran were of reduced magnitude, but normal kinetics, in rac2-/- mice, while T-dependent responses to nitrophenyl-keyhole limpet hemocyanin were subtly abnormal. Rac2 is therefore an essential element in regulating B lymphocyte functions and maintaining B lymphocyte populations in vivo.

Original languageEnglish (US)
Pages (from-to)3376-3386
Number of pages11
JournalJournal of Immunology
Volume168
Issue number7
StatePublished - Apr 1 2002
Externally publishedYes

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Antigen Receptors
Guanosine
Chemotaxis
B-Lymphocytes
Immunoglobulin M
Lymphocytosis
Peritoneal Cavity
Plasma Cells
Dextrans
Chemokines
Immunoglobulin A
Lymphocytes
Calcium
T-Lymphocytes
Serum
Population

ASJC Scopus subject areas

  • Immunology

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The Rac2 guanosine triphosphatase regulates B lymphocyte antigen receptor responses and chemotaxis and is required for establishment of B-1a and marginal zone B lymphocytes. / Croker, Ben A.; Tarlinton, David M.; Cluse, Leonie A.; Tuxen, Alana J.; Light, Amanda; Yang, Feng-Chun; Williams, David A.; Roberts, Andrew W.

In: Journal of Immunology, Vol. 168, No. 7, 01.04.2002, p. 3376-3386.

Research output: Contribution to journalArticle

Croker, Ben A. ; Tarlinton, David M. ; Cluse, Leonie A. ; Tuxen, Alana J. ; Light, Amanda ; Yang, Feng-Chun ; Williams, David A. ; Roberts, Andrew W. / The Rac2 guanosine triphosphatase regulates B lymphocyte antigen receptor responses and chemotaxis and is required for establishment of B-1a and marginal zone B lymphocytes. In: Journal of Immunology. 2002 ; Vol. 168, No. 7. pp. 3376-3386.
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