The purification of enterogastrone from porcine gut

Harold W. Lucien; Zen Itoh; David C.H. Sun; Joe Meyer; Nathaniel Carlton; Andrew V. Schally

doi:10.1016/0003-9861(69)90264-1

The purification of enterogastrone from porcine gut

Harold W. Lucien, Zen Itoh, David C.H. Sun, Joe Meyer, Nathaniel Carlton, Andrew V. Schally

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Abstract

A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.

Original language	English (US)
Pages (from-to)	180-184
Number of pages	5
Journal	Archives of Biochemistry and Biophysics
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(69)90264-1
State	Published - Oct 1969
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "The purification of enterogastrone from porcine gut",

abstract = "A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.",

author = "Lucien, {Harold W.} and Zen Itoh and Sun, {David C.H.} and Joe Meyer and Nathaniel Carlton and Schally, {Andrew V.}",

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T1 - The purification of enterogastrone from porcine gut

AU - Lucien, Harold W.

AU - Itoh, Zen

AU - Sun, David C.H.

AU - Meyer, Joe

AU - Carlton, Nathaniel

AU - Schally, Andrew V.

PY - 1969/10

Y1 - 1969/10

N2 - A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.

AB - A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.

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The purification of enterogastrone from porcine gut

Abstract

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