The purification of enterogastrone from porcine gut

Harold W. Lucien, Zen Itoh, David C.H. Sun, Joe Meyer, Nathaniel Carlton, Andrew V. Schally

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.

Original languageEnglish (US)
Pages (from-to)180-184
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume134
Issue number1
DOIs
StatePublished - Oct 1969
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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