A method is described for the purification from porcine small intestine of enterogastrone, an inhibitor of gastric secretory and motor functions. The enterogastrone activity obtained from an acetic acid extract of the duodenum was further concentrated by differential adsorption on alginic acid, elution with 0.2 m HCl, precipitation with sodium chloride and reprecipitation from aqueous ethanol with 1-butanol. Further purification of the active material was done by gel filtration on Sephadex G-50 and chromatography and rechromatography on TEAE cellulose. The enterogastrone concentrate obtained was found to be a potent inhibitor of gastric acid secretion and motility and it was not contaminated with other gastrointestinal hormones or toxic substances.
ASJC Scopus subject areas
- Molecular Biology