The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways

Kimberly L. Dodge-Kafka, Joseph Soughayer, Genevieve C. Pare, Jennifer J. Carlisle Michel, Lorene K. Langeberg, Michael S Kapiloff, John D. Scott

Research output: Contribution to journalArticle

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Abstract

Cyclic adenosine 3′, 5′-monophosphate (cAMP) is a ubiquitous mediator of intracellular signalling events. It acts principally through stimulation of cAMP-dependent protein kinases (PKAs) but also activates certain ion channels and guanine nucleotide exchange factors (Epacs). Metabolism of cAMP is catalysed by phosphodiesterases (PDEs). Here we identify a cAMP-responsive signalling complex maintained by the muscle-specific A-kinase anchoring protein (mAKAP) that includes PKA, PDE4D3 and Epac1. These intermolecular interactions facilitate the dissemination of distinct cAMP signals through each effector protein. Anchored PKA stimulates PDE4D3 to reduce local cAMP concentrations, whereas an mAKAP-associated ERK5 kinase module suppresses PDE4D3. PDE4D3 also functions as an adaptor protein that recruits Epac1, an exchange factor for the small GTPase Rap1, to enable cAMP-dependent attenuation of ERK5. Pharmacological and molecular manipulations of the mAKAP complex show that anchored ERK5 can induce cardiomyocyte hypertrophy. Thus, two coupled cAMP-dependent feedback loops are coordinated within the context of the mAKAP complex, suggesting that local control of cAMP signalling by AKAP proteins is more intricate than previously appreciated.

Original languageEnglish
Pages (from-to)574-578
Number of pages5
JournalNature
Volume437
Issue number7058
DOIs
StatePublished - Sep 22 2005
Externally publishedYes

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Muscle Proteins
Cyclic AMP-Dependent Protein Kinases
Adenosine
Protein Kinases
Muscles
Mitogen-Activated Protein Kinase 7
Guanine Nucleotide Exchange Factors
Proteins
Monomeric GTP-Binding Proteins
Phosphoric Diester Hydrolases
Ion Channels
Cardiac Myocytes
Hypertrophy
Pharmacology

ASJC Scopus subject areas

  • General

Cite this

Dodge-Kafka, K. L., Soughayer, J., Pare, G. C., Carlisle Michel, J. J., Langeberg, L. K., Kapiloff, M. S., & Scott, J. D. (2005). The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. Nature, 437(7058), 574-578. https://doi.org/10.1038/nature03966

The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. / Dodge-Kafka, Kimberly L.; Soughayer, Joseph; Pare, Genevieve C.; Carlisle Michel, Jennifer J.; Langeberg, Lorene K.; Kapiloff, Michael S; Scott, John D.

In: Nature, Vol. 437, No. 7058, 22.09.2005, p. 574-578.

Research output: Contribution to journalArticle

Dodge-Kafka, KL, Soughayer, J, Pare, GC, Carlisle Michel, JJ, Langeberg, LK, Kapiloff, MS & Scott, JD 2005, 'The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways', Nature, vol. 437, no. 7058, pp. 574-578. https://doi.org/10.1038/nature03966
Dodge-Kafka KL, Soughayer J, Pare GC, Carlisle Michel JJ, Langeberg LK, Kapiloff MS et al. The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. Nature. 2005 Sep 22;437(7058):574-578. https://doi.org/10.1038/nature03966
Dodge-Kafka, Kimberly L. ; Soughayer, Joseph ; Pare, Genevieve C. ; Carlisle Michel, Jennifer J. ; Langeberg, Lorene K. ; Kapiloff, Michael S ; Scott, John D. / The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. In: Nature. 2005 ; Vol. 437, No. 7058. pp. 574-578.
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