The functions of roughly a third of all proteins in Streptococcus pneumoniae, a significant human-pathogenic bacterium, are unknown. Using a yeast twohybrid approach, we have determined more than 2,000 novel protein interactions in this organism. We augmented this network with meta-interactome data that we defined as the pool of all interactions between evolutionarily conserved proteins in other bacteria. We found that such interactions significantly improved our ability to predict a protein's function, allowing us to provide functional predictions for 299 S. pneumoniae proteins with previously unknown functions.
- Functional prediction
- Protein-protein interactions
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Modeling and Simulation
- Molecular Biology
- Computer Science Applications