The proteasome regulates receptor-mediated endocytosis of interleukin-2

Aixin Yu, Thomas Malek

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Recent studies have increasingly implicated the proteasome in the regulation of cell surface receptors. In the present study, we investigated the role of the proteasome for ligand-dependent endocytosis and degradation of the interleukin-2 (IL-2)-interleukin-2 receptor (IL-2R) complex. Proteasome inhibitors impaired internalization of IL-2·IL-2R and prevented the lysosomal degradation of this cytokine. Based on time-course studies, proteasome activity is primarily required after initial endocytosis of the IL-2·IL-2R. Proteasome function was also necessary for the lysosomal degradation of IL-2 internalized by IL-2R that were comprised of cytoplastmic taillessm β- or γc-subunits, suggesting that the target protein for the proteasome is independent of either the cytoplasmic tail of the IL-2R β- or γc-subunits and their associated signaling components. Therefore, a functional proteasome is required for optimal endocytosis of the IL-2R/ligand complex and is essential for the subsequent lysosomal degradation of IL-2, possibly by regulating trafficking to the lysosome.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number1
DOIs
StatePublished - Jan 5 2001

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Proteasome Endopeptidase Complex
Endocytosis
Interleukin-2
Interleukin-2 Receptors
Degradation
Ligands
Time and motion study
Proteasome Inhibitors
Cell Surface Receptors
Lysosomes
Cytokines
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The proteasome regulates receptor-mediated endocytosis of interleukin-2. / Yu, Aixin; Malek, Thomas.

In: Journal of Biological Chemistry, Vol. 276, No. 1, 05.01.2001, p. 381-385.

Research output: Contribution to journalArticle

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