Abstract
Cell lines into which cloned H-2 genes had been introduced (i.e., transformants) were used to correlate the genes and their products that are capable of functioning as H-2 restriction elements for hapten-self- (AED and TNP) specific cytotoxic T cells (CTL). These transformants provided a unique system in which major histocompatibility restricted (MHC) T cell recognition could be examined by using cells that express only H-2L(d) or only H-2D(d) gene products. BALB/c[H-2(d)] anti AED-self CTL lysed both the H-2L(d) and D(d) transformants, but not parental, i.e., untransformed, cells. The AED-self lysis of the L(d) and D(d) transformants was shown to be specifically inhibited by anti-H2L(d) and anti H-2D(d) monoclonal antibody, respectively. In contrast to these results, BALB/c anti TNP-self CTL were found to lyse readily the D(d) but not L(d) transformed lines, supporting reports indicating that H-2L(d)-restricted TNP-self CTL could not be detected. The results of this study thus demonstrate that the cell surface products encoded by these transferred MHC class I genes contain self determinants recognized by CTL.
| Original language | English |
|---|---|
| Pages (from-to) | 2514-2518 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 130 |
| Issue number | 6 |
| State | Published - Aug 4 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
The products of transferred H-2 genes express determinants that restrict hapten-specific cytotoxic T cells. / Levy, Robert B; Richardson, J. C.; Margulies, D. H.; Evans, G. A.; Seidman, J. G.; Ozato, K.
In: Journal of Immunology, Vol. 130, No. 6, 04.08.1983, p. 2514-2518.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The products of transferred H-2 genes express determinants that restrict hapten-specific cytotoxic T cells
AU - Levy, Robert B
AU - Richardson, J. C.
AU - Margulies, D. H.
AU - Evans, G. A.
AU - Seidman, J. G.
AU - Ozato, K.
PY - 1983/8/4
Y1 - 1983/8/4
N2 - Cell lines into which cloned H-2 genes had been introduced (i.e., transformants) were used to correlate the genes and their products that are capable of functioning as H-2 restriction elements for hapten-self- (AED and TNP) specific cytotoxic T cells (CTL). These transformants provided a unique system in which major histocompatibility restricted (MHC) T cell recognition could be examined by using cells that express only H-2L(d) or only H-2D(d) gene products. BALB/c[H-2(d)] anti AED-self CTL lysed both the H-2L(d) and D(d) transformants, but not parental, i.e., untransformed, cells. The AED-self lysis of the L(d) and D(d) transformants was shown to be specifically inhibited by anti-H2L(d) and anti H-2D(d) monoclonal antibody, respectively. In contrast to these results, BALB/c anti TNP-self CTL were found to lyse readily the D(d) but not L(d) transformed lines, supporting reports indicating that H-2L(d)-restricted TNP-self CTL could not be detected. The results of this study thus demonstrate that the cell surface products encoded by these transferred MHC class I genes contain self determinants recognized by CTL.
AB - Cell lines into which cloned H-2 genes had been introduced (i.e., transformants) were used to correlate the genes and their products that are capable of functioning as H-2 restriction elements for hapten-self- (AED and TNP) specific cytotoxic T cells (CTL). These transformants provided a unique system in which major histocompatibility restricted (MHC) T cell recognition could be examined by using cells that express only H-2L(d) or only H-2D(d) gene products. BALB/c[H-2(d)] anti AED-self CTL lysed both the H-2L(d) and D(d) transformants, but not parental, i.e., untransformed, cells. The AED-self lysis of the L(d) and D(d) transformants was shown to be specifically inhibited by anti-H2L(d) and anti H-2D(d) monoclonal antibody, respectively. In contrast to these results, BALB/c anti TNP-self CTL were found to lyse readily the D(d) but not L(d) transformed lines, supporting reports indicating that H-2L(d)-restricted TNP-self CTL could not be detected. The results of this study thus demonstrate that the cell surface products encoded by these transferred MHC class I genes contain self determinants recognized by CTL.
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M3 - Article
VL - 130
SP - 2514
EP - 2518
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -