The primitive endoderm segregates from the epiblast in β1 integrin-deficient early mouse embryos

Robert Moore, Wensi Tao, Elizabeth R. Smith, Xiangxi Xu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We analyzed the mechanism of developmental failure in implanted β1 integrin-null blastocysts and found that primitive endoderm cells are present but segregate away from, instead of forming an epithelial layer covering, the inner cell mass. This cell segregation phenotype was also reproduced in β1 integrin-null embryoid bodies, in which primitive endoderm cells segregated and appeared as miniature aggregates detached from the core spheroids, and a primitive endoderm layer failed to form on the surface. Restricted β1 integrin gene deletion in embryos using Ttr-Cre or Sox2-Cre indicated that the loss of integrin function in the cells of the inner core rather than the outer layer is responsible for the failure to form a primitive endoderm layer. We conclude that β1 integrin is essential for the attachment of the primitive endoderm layer to the epiblast during the formation of a basement membrane, a process concurrent with the transition from cadherin- to integrin-mediated cell adhesion.

Original languageEnglish
Pages (from-to)560-572
Number of pages13
JournalMolecular and Cellular Biology
Volume34
Issue number3
DOIs
StatePublished - Feb 1 2014

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Germ Layers
Endoderm
Integrins
Embryonic Structures
Embryoid Bodies
Cell Separation
Gene Deletion
Blastocyst
Cadherins
Basement Membrane
Cell Adhesion
Phenotype

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The primitive endoderm segregates from the epiblast in β1 integrin-deficient early mouse embryos. / Moore, Robert; Tao, Wensi; Smith, Elizabeth R.; Xu, Xiangxi.

In: Molecular and Cellular Biology, Vol. 34, No. 3, 01.02.2014, p. 560-572.

Research output: Contribution to journalArticle

Moore, Robert ; Tao, Wensi ; Smith, Elizabeth R. ; Xu, Xiangxi. / The primitive endoderm segregates from the epiblast in β1 integrin-deficient early mouse embryos. In: Molecular and Cellular Biology. 2014 ; Vol. 34, No. 3. pp. 560-572.
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