The primary responses of murine neonatal lymph node CD4+ cells are Th2-skewed and are sufficient for the development of Th2-biased memory

Rebecca D Adkins, Yurong Bu, Vladimir Vincek, Patricia Guevara

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Exposure of neonatal mice to antigen often results in Th2-biased responses in later life. Examples of this Th2 tendency are (a) secondary antibody responses dominated by the Th2-associated IgG1 isotype and (b) Th2-mediated tolerance to alloantigens. We previously reported that neonates develop primary Th1 and Th2 function in the lymph nodes but exclusive Th2 primary splenic responses. Here, we have tested whether the Th2 bias of adults initially immunized as neonates is due to the early, primary Th2 polarization in the spleen. Surprisingly, removal of the spleen at birth had no affect on either IgG1-dominant secondary responses or the development of tolerance to alloantigens. Thus, neonatal lymph nodes are sufficient to generate Th2-biased function following neonatal antigen exposure. To understand how this could arise, we examined the primary Th1/Th2 responses of CD4+ lymph node cells. Unlike the balanced Th1/Th2 responses seen with total lymph node cells, the primary responses of isolated CD4+ cells were skewed to IL-4 producing function. These results suggest that the early development of Th2-dominant responses by lymph node CD4+ cells contributes substantially to the subsequent development of Th2-dominant memory in neonates.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalClinical and Developmental Immunology
Volume10
Issue number1
DOIs
StatePublished - Mar 1 2003

Fingerprint

Th2 Cells
Lymph Nodes
Isoantigens
Spleen
Immunoglobulin G
Antigens
Interleukin-4
Antibody Formation
Parturition

Keywords

  • Memory
  • Ontogeny(neonatal)
  • Spleen and lymph nodes
  • Th1/Th2
  • Tolerance

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Developmental Biology

Cite this

The primary responses of murine neonatal lymph node CD4+ cells are Th2-skewed and are sufficient for the development of Th2-biased memory. / Adkins, Rebecca D; Bu, Yurong; Vincek, Vladimir; Guevara, Patricia.

In: Clinical and Developmental Immunology, Vol. 10, No. 1, 01.03.2003, p. 43-51.

Research output: Contribution to journalArticle

@article{34bafa95504f46de8d18f0740d754c96,
title = "The primary responses of murine neonatal lymph node CD4+ cells are Th2-skewed and are sufficient for the development of Th2-biased memory",
abstract = "Exposure of neonatal mice to antigen often results in Th2-biased responses in later life. Examples of this Th2 tendency are (a) secondary antibody responses dominated by the Th2-associated IgG1 isotype and (b) Th2-mediated tolerance to alloantigens. We previously reported that neonates develop primary Th1 and Th2 function in the lymph nodes but exclusive Th2 primary splenic responses. Here, we have tested whether the Th2 bias of adults initially immunized as neonates is due to the early, primary Th2 polarization in the spleen. Surprisingly, removal of the spleen at birth had no affect on either IgG1-dominant secondary responses or the development of tolerance to alloantigens. Thus, neonatal lymph nodes are sufficient to generate Th2-biased function following neonatal antigen exposure. To understand how this could arise, we examined the primary Th1/Th2 responses of CD4+ lymph node cells. Unlike the balanced Th1/Th2 responses seen with total lymph node cells, the primary responses of isolated CD4+ cells were skewed to IL-4 producing function. These results suggest that the early development of Th2-dominant responses by lymph node CD4+ cells contributes substantially to the subsequent development of Th2-dominant memory in neonates.",
keywords = "Memory, Ontogeny(neonatal), Spleen and lymph nodes, Th1/Th2, Tolerance",
author = "Adkins, {Rebecca D} and Yurong Bu and Vladimir Vincek and Patricia Guevara",
year = "2003",
month = "3",
day = "1",
doi = "10.1080/10446670310001598474",
language = "English",
volume = "10",
pages = "43--51",
journal = "Journal of Immunology Research",
issn = "2314-8861",
publisher = "Hindawi Publishing Corporation",
number = "1",

}

TY - JOUR

T1 - The primary responses of murine neonatal lymph node CD4+ cells are Th2-skewed and are sufficient for the development of Th2-biased memory

AU - Adkins, Rebecca D

AU - Bu, Yurong

AU - Vincek, Vladimir

AU - Guevara, Patricia

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Exposure of neonatal mice to antigen often results in Th2-biased responses in later life. Examples of this Th2 tendency are (a) secondary antibody responses dominated by the Th2-associated IgG1 isotype and (b) Th2-mediated tolerance to alloantigens. We previously reported that neonates develop primary Th1 and Th2 function in the lymph nodes but exclusive Th2 primary splenic responses. Here, we have tested whether the Th2 bias of adults initially immunized as neonates is due to the early, primary Th2 polarization in the spleen. Surprisingly, removal of the spleen at birth had no affect on either IgG1-dominant secondary responses or the development of tolerance to alloantigens. Thus, neonatal lymph nodes are sufficient to generate Th2-biased function following neonatal antigen exposure. To understand how this could arise, we examined the primary Th1/Th2 responses of CD4+ lymph node cells. Unlike the balanced Th1/Th2 responses seen with total lymph node cells, the primary responses of isolated CD4+ cells were skewed to IL-4 producing function. These results suggest that the early development of Th2-dominant responses by lymph node CD4+ cells contributes substantially to the subsequent development of Th2-dominant memory in neonates.

AB - Exposure of neonatal mice to antigen often results in Th2-biased responses in later life. Examples of this Th2 tendency are (a) secondary antibody responses dominated by the Th2-associated IgG1 isotype and (b) Th2-mediated tolerance to alloantigens. We previously reported that neonates develop primary Th1 and Th2 function in the lymph nodes but exclusive Th2 primary splenic responses. Here, we have tested whether the Th2 bias of adults initially immunized as neonates is due to the early, primary Th2 polarization in the spleen. Surprisingly, removal of the spleen at birth had no affect on either IgG1-dominant secondary responses or the development of tolerance to alloantigens. Thus, neonatal lymph nodes are sufficient to generate Th2-biased function following neonatal antigen exposure. To understand how this could arise, we examined the primary Th1/Th2 responses of CD4+ lymph node cells. Unlike the balanced Th1/Th2 responses seen with total lymph node cells, the primary responses of isolated CD4+ cells were skewed to IL-4 producing function. These results suggest that the early development of Th2-dominant responses by lymph node CD4+ cells contributes substantially to the subsequent development of Th2-dominant memory in neonates.

KW - Memory

KW - Ontogeny(neonatal)

KW - Spleen and lymph nodes

KW - Th1/Th2

KW - Tolerance

UR - http://www.scopus.com/inward/record.url?scp=0141960151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141960151&partnerID=8YFLogxK

U2 - 10.1080/10446670310001598474

DO - 10.1080/10446670310001598474

M3 - Article

C2 - 14575157

AN - SCOPUS:0141960151

VL - 10

SP - 43

EP - 51

JO - Journal of Immunology Research

JF - Journal of Immunology Research

SN - 2314-8861

IS - 1

ER -