The presence of three repeats in the 5′ UTR region of thymidylate synthase (TS) is associated with increased TS mRNA expression in cultured human cancer cell lines in vitro

Hiba Hammad, Malancha Sarkar, Nikhil Gupta, Bach Ardalan, Subbayan Pochi

Research output: Contribution to journalArticle

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Abstract

Thymidylate synthase (TS) gene contains 28-bp polymorphic sequence and 6-bp insert at the 5′- and 3′-untranslated region, respectively. We investigated the role of these two polymorphic traits on TS mRNA expression in nine different cultured human cancer cell lines in vitro. Three cell lines each were 2R/2R, 2R/3R and 3R/3R genotypes. Six of the nine cell lines tested homozygous for the presence of 6-bp insert (+6/+6) and the rest three lacked this insert (-6/-6). TS expression analyses associated homozygous three repeats (3R/3R) to higher TS expression.

Original languageEnglish
Pages (from-to)246-249
Number of pages4
JournalOncology Reports
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Thymidylate Synthase
5' Untranslated Regions
Cell Line
Messenger RNA
Neoplasms
3' Untranslated Regions
Genotype
In Vitro Techniques
Genes

Keywords

  • 3′ UTR insertion
  • Genotypic analysis
  • Real-time PCR
  • Thymidylate synthase mRNA level
  • Translation regulation
  • TS enhancer region

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The presence of three repeats in the 5′ UTR region of thymidylate synthase (TS) is associated with increased TS mRNA expression in cultured human cancer cell lines in vitro. / Hammad, Hiba; Sarkar, Malancha; Gupta, Nikhil; Ardalan, Bach; Pochi, Subbayan.

In: Oncology Reports, Vol. 27, No. 1, 01.01.2012, p. 246-249.

Research output: Contribution to journalArticle

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