The presence of three repeats in the 5′ UTR region of thymidylate synthase (TS) is associated with increased TS mRNA expression in cultured human cancer cell lines in vitro

Hiba Hammad, Malancha Sarkar, Nikhil Gupta, Bach Ardalan, Pochi R. Subbarayan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Thymidylate synthase (TS) gene contains 28-bp polymorphic sequence and 6-bp insert at the 5′- and 3′-untranslated region, respectively. We investigated the role of these two polymorphic traits on TS mRNA expression in nine different cultured human cancer cell lines in vitro. Three cell lines each were 2R/2R, 2R/3R and 3R/3R genotypes. Six of the nine cell lines tested homozygous for the presence of 6-bp insert (+6/+6) and the rest three lacked this insert (-6/-6). TS expression analyses associated homozygous three repeats (3R/3R) to higher TS expression.

Original languageEnglish (US)
Pages (from-to)246-249
Number of pages4
JournalOncology Reports
Volume27
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • 3′ UTR insertion
  • Genotypic analysis
  • Real-time PCR
  • Thymidylate synthase mRNA level
  • Translation regulation
  • TS enhancer region

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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