The presence of LHRH-like receptors in Dunning R3327H prostate tumors

Marion T. Hierowski, Altamirano Perla, Tommie W. Redding, Andrew V. Schally

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Quantitative analyses of LH-RH-like membrane receptors were performed in five tumors from the transplantable Dunning R3372H rat prostatic adenocarcinoma. The binding of D-Trp6-LH-RH, an agonist of LH-RH, was observed in all 5 tumors. The antagonist [Ac-Dp-Cl-Phe1,2,D-Trp3,D-Lys6,D-Ala10]-LH-RH was bound to 4 tumors. The apparent equilibrium dissociation constant (Kd) for D-Trp6-LH-RH receptor was from 2.6-3.9 × 10-10 M. The apparent equilibrium Bmax values (maximum number of binding sites) were from 17.2-86.0 fmol/mg membrane protein for D-Trp6-LH-RH receptor. The Kd for the antagonist was from 2.4-2.7 × 10-10 M and the Bmax values were from 35.5-66.0 fmol/mg membrane protein. Similar binding studies performed in 6 normal rat prostates showed no binding capacities.

Original languageEnglish (US)
Pages (from-to)92-96
Number of pages5
JournalFEBS letters
Volume154
Issue number1
DOIs
StatePublished - Apr 5 1983
Externally publishedYes

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Keywords

  • Dunning R3327H prostate tumor
  • LH-RH analog binding
  • Membrane protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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