The presence of LHRH-like receptors in Dunning R3327H prostate tumors

Marion T. Hierowski, Altamirano Perla, Tommie W. Redding, Andrew V Schally

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Quantitative analyses of LH-RH-like membrane receptors were performed in five tumors from the transplantable Dunning R3372H rat prostatic adenocarcinoma. The binding of D-Trp6-LH-RH, an agonist of LH-RH, was observed in all 5 tumors. The antagonist [Ac-Dp-Cl-Phe1,2,D-Trp3,D-Lys6,D-Ala10]-LH-RH was bound to 4 tumors. The apparent equilibrium dissociation constant (Kd) for D-Trp6-LH-RH receptor was from 2.6-3.9 × 10-10 M. The apparent equilibrium Bmax values (maximum number of binding sites) were from 17.2-86.0 fmol/mg membrane protein for D-Trp6-LH-RH receptor. The Kd for the antagonist was from 2.4-2.7 × 10-10 M and the Bmax values were from 35.5-66.0 fmol/mg membrane protein. Similar binding studies performed in 6 normal rat prostates showed no binding capacities.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalFEBS Letters
Volume154
Issue number1
DOIs
StatePublished - Apr 5 1983
Externally publishedYes

Fingerprint

LHRH Receptors
Gonadotropin-Releasing Hormone
Tumors
Prostate
LH Receptors
Neoplasms
Rats
Membrane Proteins
Adenocarcinoma
Binding Sites
Membranes

Keywords

  • Dunning R3327H prostate tumor
  • LH-RH analog binding
  • Membrane protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The presence of LHRH-like receptors in Dunning R3327H prostate tumors. / Hierowski, Marion T.; Perla, Altamirano; Redding, Tommie W.; Schally, Andrew V.

In: FEBS Letters, Vol. 154, No. 1, 05.04.1983, p. 92-96.

Research output: Contribution to journalArticle

Hierowski, Marion T. ; Perla, Altamirano ; Redding, Tommie W. ; Schally, Andrew V. / The presence of LHRH-like receptors in Dunning R3327H prostate tumors. In: FEBS Letters. 1983 ; Vol. 154, No. 1. pp. 92-96.
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