TY - JOUR
T1 - The pregnancy-specific glycoprotein family of the immunoglobulin superfamily
T2 - Identification of new members and estimation of family size
AU - Khan, Wasif Noor
AU - Teglund, Stephan
AU - Bremer, Kåre
AU - Hammarström, Sten
N1 - Funding Information:
The skillful technical and secretarial assistance of Ms. Anne Israelsson and Ms. Christine BostrSm, respectively, is gratefully acknowledged. The work was supported by grants from the Swedish Natural Science Research Council, the Swedish Cancer Society, Tor-sten och Ragnar Siiderbergs stiftelser, and J. C. Kempes Minnes Sti-pendiefond.
PY - 1992/4
Y1 - 1992/4
N2 - The members of the carcinoembryonic antigen (CEA)/pregnancy-specific glycoprotein (PSG) gene family have a characteristic N-terminal domain that is homologous to the immunoglobulin variable region. We have estimated the size of the PSG subfamily by identification of N-domain exons from isolated genomic clones and from total genomic DNA through PCR amplification and DNA sequence determination. The PSG subfamily contains at least 11 different genes. For 7 of these, two DNA sequences differing from each other in 1 to 4 nucleotides were detected. Most likely, they represent different alleles. They are PSG1, PSG2, PSG3, PSG4, PSG5, PSG6, PSG7, PSG8, PSG11, PSG12, and PSG13. Six of the N-domain sequences described here are new. All of the PSGs except PSG1, PSG4, and PSG8 contained the arginine-glycine-aspartic acid sequence at position 93-95 corresponding to the complementarity determining region 3 of immunoglobulin. Parsimony analysis of 24 CEA and PSG sequences using 12 members of the immunoglobulin gene superfamily as out-groups to root the family free shows that the N-domain of the CEA group genes evolved in one major branch and the PSG group genes in the other.
AB - The members of the carcinoembryonic antigen (CEA)/pregnancy-specific glycoprotein (PSG) gene family have a characteristic N-terminal domain that is homologous to the immunoglobulin variable region. We have estimated the size of the PSG subfamily by identification of N-domain exons from isolated genomic clones and from total genomic DNA through PCR amplification and DNA sequence determination. The PSG subfamily contains at least 11 different genes. For 7 of these, two DNA sequences differing from each other in 1 to 4 nucleotides were detected. Most likely, they represent different alleles. They are PSG1, PSG2, PSG3, PSG4, PSG5, PSG6, PSG7, PSG8, PSG11, PSG12, and PSG13. Six of the N-domain sequences described here are new. All of the PSGs except PSG1, PSG4, and PSG8 contained the arginine-glycine-aspartic acid sequence at position 93-95 corresponding to the complementarity determining region 3 of immunoglobulin. Parsimony analysis of 24 CEA and PSG sequences using 12 members of the immunoglobulin gene superfamily as out-groups to root the family free shows that the N-domain of the CEA group genes evolved in one major branch and the PSG group genes in the other.
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U2 - 10.1016/0888-7543(92)90309-G
DO - 10.1016/0888-7543(92)90309-G
M3 - Article
C2 - 1572651
AN - SCOPUS:0026544591
VL - 12
SP - 780
EP - 787
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 4
ER -