The potassium channel subunit Kvβ3 interacts with pannexin 1 and attenuates its sensitivity to changes in redox potentials

Stefanie Bunse, Silviu Locovei, Matthias Schmidt, Feng Qiu, Georg Zoidl, Gerhard Dahl, Rolf Dermietzel

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Pannexin 1 (Panx1), a member of the second gap junction protein family identified in vertebrates, appears to preferentially form non-junctional membrane channels. A candidate regulatory protein of Panx1 is the potassium channel subunit Kvβ3, previously identified by bacterial two-hybrid strategies. Here, we report on the physical association of Panx1 with Kvβ3 by immunoprecipitation when co-expressed in a neuroblastoma cell line (Neuro2A). Furthermore, in vivo co-expression of Panx1 and Kvβ3 was shown to occur in murine hippocampus and cerebellum. Kvβ3 is known to accelerate inactivation of otherwise slowly inactivating potassium channels under reducing conditions. We subsequently found that Panx1 channel currents exhibit a significant reduction when exposed to reducing agents, and that this effect is attenuated in the presence of Kvβ3. Apparently, Kvβ3 is involved in regulating the susceptibility of Panx1 channels to redox potential. Furthermore, the Panx1 channel blockers carbenoxolone and Probenecid were less effective in inhibiting Panx1 currents when Kvβ3 was co-expressed. The influence of Kvβ3 on Panx1 is the first example of modulation of Panx1 channel function(s) by interacting proteins, and suggests the physiological importance of sensing changes in redox potentials.

Original languageEnglish (US)
Pages (from-to)6258-6270
Number of pages13
JournalFEBS Journal
Volume276
Issue number21
DOIs
StatePublished - Nov 1 2009

Keywords

  • Channel
  • Drug sensitivity
  • Interacting proteins
  • Modulation
  • Potassium channel subunit

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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