Abstract
In the present work we investigate the structural organization of the nucleoskeleton of Allium cepa meristematic root cells. Resinless sections reveal for the first time a residual filamentous network in plant nuclei. This network is composed of branched knobbed filaments with associated globular structures, connected to the lamina and to the dense aggregates of different sizes. Results of immunoblotting show that many components of this network are homologues of intermediate filament-type proteins. NUMA, a coiled-coil protein related to intermediate filaments, found in animal cells, can also be detected in this plant nuclear matrix system. Immunofluorescence reveals a diffuse distribution of the animal NuMA homologues in plant nuclear core filaments in interphase. Resinless immunoelectron microscopy further reveals a distribution along the extended filaments and the dense aggregates. During mitosis, in contrast to the accumulation at the poles in animal cells, NuMA homologues in plant onion cells show a diffuse pattern, which may correspond to the spindle matrix. Our data are the first report of the conservation in plants of NuMA proteins, which may be involved in both nuclear and mitotic spindle organizations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 516-526 |
| Number of pages | 11 |
| Journal | Experimental Cell Research |
| Volume | 246 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 1999 |
| Externally published | Yes |
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Keywords
- Immunoblotting
- Immunofluorescence
- Immunogold
- NuMA
- Plant nuclear matrix
- Resinless sections
ASJC Scopus subject areas
- Cell Biology
Cite this
The plant nucleoskeleton : Ultrastructural organization and identification of NuMa homologues in the nuclear matrix and mitotic spindle of plant cells. / Yu, Wendou; Moreno Díaz De La Espina, Susana.
In: Experimental Cell Research, Vol. 246, No. 2, 01.02.1999, p. 516-526.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The plant nucleoskeleton
T2 - Ultrastructural organization and identification of NuMa homologues in the nuclear matrix and mitotic spindle of plant cells
AU - Yu, Wendou
AU - Moreno Díaz De La Espina, Susana
PY - 1999/2/1
Y1 - 1999/2/1
N2 - In the present work we investigate the structural organization of the nucleoskeleton of Allium cepa meristematic root cells. Resinless sections reveal for the first time a residual filamentous network in plant nuclei. This network is composed of branched knobbed filaments with associated globular structures, connected to the lamina and to the dense aggregates of different sizes. Results of immunoblotting show that many components of this network are homologues of intermediate filament-type proteins. NUMA, a coiled-coil protein related to intermediate filaments, found in animal cells, can also be detected in this plant nuclear matrix system. Immunofluorescence reveals a diffuse distribution of the animal NuMA homologues in plant nuclear core filaments in interphase. Resinless immunoelectron microscopy further reveals a distribution along the extended filaments and the dense aggregates. During mitosis, in contrast to the accumulation at the poles in animal cells, NuMA homologues in plant onion cells show a diffuse pattern, which may correspond to the spindle matrix. Our data are the first report of the conservation in plants of NuMA proteins, which may be involved in both nuclear and mitotic spindle organizations.
AB - In the present work we investigate the structural organization of the nucleoskeleton of Allium cepa meristematic root cells. Resinless sections reveal for the first time a residual filamentous network in plant nuclei. This network is composed of branched knobbed filaments with associated globular structures, connected to the lamina and to the dense aggregates of different sizes. Results of immunoblotting show that many components of this network are homologues of intermediate filament-type proteins. NUMA, a coiled-coil protein related to intermediate filaments, found in animal cells, can also be detected in this plant nuclear matrix system. Immunofluorescence reveals a diffuse distribution of the animal NuMA homologues in plant nuclear core filaments in interphase. Resinless immunoelectron microscopy further reveals a distribution along the extended filaments and the dense aggregates. During mitosis, in contrast to the accumulation at the poles in animal cells, NuMA homologues in plant onion cells show a diffuse pattern, which may correspond to the spindle matrix. Our data are the first report of the conservation in plants of NuMA proteins, which may be involved in both nuclear and mitotic spindle organizations.
KW - Immunoblotting
KW - Immunofluorescence
KW - Immunogold
KW - NuMA
KW - Plant nuclear matrix
KW - Resinless sections
UR - http://www.scopus.com/inward/record.url?scp=0344631628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344631628&partnerID=8YFLogxK
U2 - 10.1006/excr.1998.4334
DO - 10.1006/excr.1998.4334
M3 - Article
C2 - 9925768
AN - SCOPUS:0344631628
VL - 246
SP - 516
EP - 526
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -