Abstract
The pigtail macaque (Macaca nemestrina) is a common model for the study of AIDS. The pigtail major histocompatibility complex class I allele Mane-A*10 restricts an immunodominant simian immunodeficiency virus (SIV) Gag epitope (KP9) which rapidly mutates to escape T cell recognition following acute simian/human immunodeficiency virus infection. Two technologies for the detection of Mane-A*10 in outbred pigtail macaques were developed: reference strand-mediated conformational analysis and sequence-specific primer polymerase chain reaction. A Mane-A*10/K-P9 tetramer was then developed to quantify CD8+ T lymphocytes primed by multigenic DNA vaccination, which have previously been difficult to detect using standard interferon-γ-based T cell assays. We also demonstrate mutational escape at KP9 following acute SIV infection. Mane-A*10+ animals have lower set point SIV levels than Mane-A*10- animals, suggesting a significant fitness cost of escape. These studies pave the way for a more robust understanding of HIV vaccines in pigtail macaques.
Original language | English (US) |
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Pages (from-to) | 282-293 |
Number of pages | 12 |
Journal | Journal of medical primatology |
Volume | 34 |
Issue number | 5-6 SPEC. ISS. |
DOIs | |
State | Published - Oct 2005 |
Externally published | Yes |
Keywords
- CD8 T cells
- Major histocompatibility complex typing
- Tetramer
- Vaccines
- Viral escape
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)