The physiology of massive zinc accumulation in the liver of female squirrelfish and its relationship to reproduction

Christer Hogstrand, Nancy J. Gassman, Bilyana Popova, Chris M. Wood, Patrick J. Walsh

Research output: Contribution to journalReview article

25 Scopus citations

Abstract

It is well known that zinc is an essential micronutrient and, as a rule, organisms keep relatively constant low levels of zinc to maintain cellular functions. The squirrelfish family (Holocentridae) is the only known exception from this rule. Squirrelfish accumulate very high concentrations of zinc in the liver. In the present study, we demonstrate that, while female squirrelfish store large amounts of zinc in the liver and ovaries, the males show zinc levels that are typical for vertebrates. The zinc content of the diet is the same in males and females, and zinc is not lost from the liver during starvation. Thus, the difference between genders in zinc storage is not dependent upon the diet. Rather, there are at least two processes that contribute to the accumulation in females. First, females possess high levels of two major zinc-binding proteins: metallothionein (MT) and a novel female- specific zinc-binding protein (FZnBP). In females, but not in males, almost all MT is present in the hepatocyte nucleus. FZnBP is exclusively found in the hepatocyte cytosol of females. Second, hepatocytes of female squirrelfish have a high capacity to transport zinc across the plasma membrane. In addition to the liver, only the gonads of females showed unusually high concentrations of zinc. Administration of exogenous oestrogen to females decreases the hepatic zinc concentration while there is a matching increase in the zinc content of the ovaries. Thus, oestrogen may trigger a redistribution of zinc from liver to ovaries. Together, our findings suggest that female squirrelfish may be uniquely adapted to detoxify zinc and to utilize it as a macronutrient for processes related to reproduction.

Original languageEnglish (US)
Pages (from-to)2543-2554
Number of pages12
JournalJournal of Experimental Biology
Volume199
Issue number11
StatePublished - Nov 1 1996

Keywords

  • fish
  • FZnBP
  • Holocentrus marianus
  • metallothionein
  • physiology
  • pisces
  • reproduction
  • squirrelfish
  • zinc
  • zinc accumulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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    Hogstrand, C., Gassman, N. J., Popova, B., Wood, C. M., & Walsh, P. J. (1996). The physiology of massive zinc accumulation in the liver of female squirrelfish and its relationship to reproduction. Journal of Experimental Biology, 199(11), 2543-2554.