TY - JOUR
T1 - The peripheral benzodiazepine receptor and neurosteroids in hepatic encephalopathy
AU - Norenberg, Michael D.
AU - Itzhak, Yossef
AU - Bender, Alex S.
PY - 1997
Y1 - 1997
N2 - Ammonia and astrocytes are inextricably involved in the mechanism of HE. We propose that ammonia, by upregulating the astrocytic peripheral benzodiazepine receptor, results in the production of neurosteroids that exert a positive modulatory effect on the GABA(A) receptor which contributes to the neuroinhibition and neurologic dysfunction associated with hepatic encephalopathy. In support of this hypothesis, we, and others, have shown: 1) that the PBR is upregulated in animal models of hyperammonemia/HE as well as in ammonia-treated astrocyte cultures; 2) that PK 11195, a specific blocker of the PBR, ameliorates ammonia toxicity in vivo; 3) that the synthesis of pregnenolone, the parent compound for all neurosteroids, is increased in ammonia-treated astrocyte cultures and in in vivo models of HE; 4) that levels of THP and THDOC, pregnenolone-derived neurosteroids with positive modulatory effects on the GABA(A) receptor, are elevated in animal models of HE; and 5) neurosteroids exert behavioral and neuropathologic changes similar to HE. This hypothesis integrates the two currently dominant pathogenetic vies of HE, namely, ammonia neurotoxicity and excessive GABAergic tone. The PBR/neurosteroid hypothesis also incorporates the pathogenetic role of astrocytes, the cells in brain principally affected in HE. The presence of the PBR on astrocytes as well as the identification of astrocytes as major sources of neurosteroids serves again to highlight their critical role in the pathogenesis of HE, a disorder which we have reviewed for some time as a primary gliopathy. The potential role of PBR upregulation resulting in increased neurosteroid production offers the potential for exciting novel therapeutic approaches to HE through modulation of neurosteroid effects.
AB - Ammonia and astrocytes are inextricably involved in the mechanism of HE. We propose that ammonia, by upregulating the astrocytic peripheral benzodiazepine receptor, results in the production of neurosteroids that exert a positive modulatory effect on the GABA(A) receptor which contributes to the neuroinhibition and neurologic dysfunction associated with hepatic encephalopathy. In support of this hypothesis, we, and others, have shown: 1) that the PBR is upregulated in animal models of hyperammonemia/HE as well as in ammonia-treated astrocyte cultures; 2) that PK 11195, a specific blocker of the PBR, ameliorates ammonia toxicity in vivo; 3) that the synthesis of pregnenolone, the parent compound for all neurosteroids, is increased in ammonia-treated astrocyte cultures and in in vivo models of HE; 4) that levels of THP and THDOC, pregnenolone-derived neurosteroids with positive modulatory effects on the GABA(A) receptor, are elevated in animal models of HE; and 5) neurosteroids exert behavioral and neuropathologic changes similar to HE. This hypothesis integrates the two currently dominant pathogenetic vies of HE, namely, ammonia neurotoxicity and excessive GABAergic tone. The PBR/neurosteroid hypothesis also incorporates the pathogenetic role of astrocytes, the cells in brain principally affected in HE. The presence of the PBR on astrocytes as well as the identification of astrocytes as major sources of neurosteroids serves again to highlight their critical role in the pathogenesis of HE, a disorder which we have reviewed for some time as a primary gliopathy. The potential role of PBR upregulation resulting in increased neurosteroid production offers the potential for exciting novel therapeutic approaches to HE through modulation of neurosteroid effects.
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U2 - 10.1007/978-1-4615-5945-0_7
DO - 10.1007/978-1-4615-5945-0_7
M3 - Article
C2 - 9286429
AN - SCOPUS:0030833288
VL - 420
SP - 95
EP - 111
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -