The outer membrane protein A (OmpA) of Yersinia pestis promotes intracellular survival and virulence in mice

Sara Schesser Bartra, Xin Gong, Cherish D. Lorica, Chaitanya Jain, Manoj K M Nair, Dieter Schifferli, Lianfen Qian, Zhongwei Li, Gregory V Plano, Kurt Schesser

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The plague bacterium Yersinia pestis has a number of well-described strategies to protect itself from both host cells and soluble factors. In an effort to identify additional anti-host factors, we employed a transposon site hybridization (TraSH)-based approach to screen 10 5 Y. pestis mutants in an in vitro infection system. In addition to loci encoding various components of the well-characterized type III secretion system (T3SS), our screen unambiguously identified ompA as a pro-survival gene. We go on to show that an engineered Y. pestis ΔompA strain, as well as a ΔompA strain of the closely related pathogen Yersinia pseudotuberculosis, have fully functioning T3SSs but are specifically defective in surviving within macrophages. Additionally, the Y. pestis ΔompA strain was out competed by the wild-type strain in a mouse co-infection assay. Unlike in other bacterial pathogens in which OmpA can promote adherence, invasion, or serum resistance, the OmpA of Y. pestis is restricted to enhancing intracellular survival. Our data show that OmpA of the pathogenic Yersinia is a virulence factor on par with the T3SS.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalMicrobial Pathogenesis
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Yersinia pestis
Virulence
Yersinia pseudotuberculosis
Yersinia
Plague
Virulence Factors
Coinfection
Macrophages
OMPA outer membrane proteins
Bacteria
Infection
Serum
Genes

Keywords

  • Intracellular
  • OmpA
  • Pathogenesis
  • Virulence
  • Yersinia pestis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

The outer membrane protein A (OmpA) of Yersinia pestis promotes intracellular survival and virulence in mice. / Bartra, Sara Schesser; Gong, Xin; Lorica, Cherish D.; Jain, Chaitanya; Nair, Manoj K M; Schifferli, Dieter; Qian, Lianfen; Li, Zhongwei; Plano, Gregory V; Schesser, Kurt.

In: Microbial Pathogenesis, Vol. 52, No. 1, 01.01.2012, p. 41-46.

Research output: Contribution to journalArticle

Bartra, Sara Schesser ; Gong, Xin ; Lorica, Cherish D. ; Jain, Chaitanya ; Nair, Manoj K M ; Schifferli, Dieter ; Qian, Lianfen ; Li, Zhongwei ; Plano, Gregory V ; Schesser, Kurt. / The outer membrane protein A (OmpA) of Yersinia pestis promotes intracellular survival and virulence in mice. In: Microbial Pathogenesis. 2012 ; Vol. 52, No. 1. pp. 41-46.
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