The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions

Ana M C Faria, Agata Levay, Yaming Wang, Alice O. Kamphorst, Magda L P Rosa, Daniel R. Nussenzveig, Wayne E Balkan, Yuh Min Chook, David E. Levy, Beatriz M A Fontoura

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96+/- mice that express low levels of Nup96, as Nup96-/- mice are lethal. The Nup96+/- mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN α- and γ-mediated induction of MHC I and IFNγ induction of MHC II, ICAM-1, and other proteins. Frequency of TCRαβ+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96+/- mice. Upon immunization, Nup96+/- mice showed impaired antigen presentation and T cell proliferation. Nup96+/- cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.

Original languageEnglish
Pages (from-to)295-304
Number of pages10
JournalImmunity
Volume24
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

Interferons
Proteins
nuclear pore complex protein 96
T-Lymphocytes
Cell Nucleus Active Transport
Antigen Presentation
Adaptive Immunity
Virus Diseases
Intercellular Adhesion Molecule-1
Innate Immunity
Immune System
Immunization
Down-Regulation
Cell Proliferation
Viruses
Messenger RNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Faria, A. M. C., Levay, A., Wang, Y., Kamphorst, A. O., Rosa, M. L. P., Nussenzveig, D. R., ... Fontoura, B. M. A. (2006). The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions. Immunity, 24(3), 295-304. https://doi.org/10.1016/j.immuni.2006.01.014

The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions. / Faria, Ana M C; Levay, Agata; Wang, Yaming; Kamphorst, Alice O.; Rosa, Magda L P; Nussenzveig, Daniel R.; Balkan, Wayne E; Chook, Yuh Min; Levy, David E.; Fontoura, Beatriz M A.

In: Immunity, Vol. 24, No. 3, 01.03.2006, p. 295-304.

Research output: Contribution to journalArticle

Faria, AMC, Levay, A, Wang, Y, Kamphorst, AO, Rosa, MLP, Nussenzveig, DR, Balkan, WE, Chook, YM, Levy, DE & Fontoura, BMA 2006, 'The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions', Immunity, vol. 24, no. 3, pp. 295-304. https://doi.org/10.1016/j.immuni.2006.01.014
Faria AMC, Levay A, Wang Y, Kamphorst AO, Rosa MLP, Nussenzveig DR et al. The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions. Immunity. 2006 Mar 1;24(3):295-304. https://doi.org/10.1016/j.immuni.2006.01.014
Faria, Ana M C ; Levay, Agata ; Wang, Yaming ; Kamphorst, Alice O. ; Rosa, Magda L P ; Nussenzveig, Daniel R. ; Balkan, Wayne E ; Chook, Yuh Min ; Levy, David E. ; Fontoura, Beatriz M A. / The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions. In: Immunity. 2006 ; Vol. 24, No. 3. pp. 295-304.
@article{7257ef326d9948e380d90175dc473e8c,
title = "The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions",
abstract = "Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96+/- mice that express low levels of Nup96, as Nup96-/- mice are lethal. The Nup96+/- mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN α- and γ-mediated induction of MHC I and IFNγ induction of MHC II, ICAM-1, and other proteins. Frequency of TCRαβ+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96+/- mice. Upon immunization, Nup96+/- mice showed impaired antigen presentation and T cell proliferation. Nup96+/- cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.",
author = "Faria, {Ana M C} and Agata Levay and Yaming Wang and Kamphorst, {Alice O.} and Rosa, {Magda L P} and Nussenzveig, {Daniel R.} and Balkan, {Wayne E} and Chook, {Yuh Min} and Levy, {David E.} and Fontoura, {Beatriz M A}",
year = "2006",
month = "3",
day = "1",
doi = "10.1016/j.immuni.2006.01.014",
language = "English",
volume = "24",
pages = "295--304",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions

AU - Faria, Ana M C

AU - Levay, Agata

AU - Wang, Yaming

AU - Kamphorst, Alice O.

AU - Rosa, Magda L P

AU - Nussenzveig, Daniel R.

AU - Balkan, Wayne E

AU - Chook, Yuh Min

AU - Levy, David E.

AU - Fontoura, Beatriz M A

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96+/- mice that express low levels of Nup96, as Nup96-/- mice are lethal. The Nup96+/- mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN α- and γ-mediated induction of MHC I and IFNγ induction of MHC II, ICAM-1, and other proteins. Frequency of TCRαβ+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96+/- mice. Upon immunization, Nup96+/- mice showed impaired antigen presentation and T cell proliferation. Nup96+/- cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.

AB - Nup98 and Nup96 are components of the nuclear transport machinery and are induced by interferons (IFN). Nup98 is a constituent of an mRNA export pathway that is targeted by viruses and regulated by IFN. However, the role of Nup96 in IFN-related mechanisms has not been established. To investigate the function of Nup96 in vivo, we generated Nup96+/- mice that express low levels of Nup96, as Nup96-/- mice are lethal. The Nup96+/- mice presented selective alterations of the immune system, which resulted in downregulation and impaired IFN α- and γ-mediated induction of MHC I and IFNγ induction of MHC II, ICAM-1, and other proteins. Frequency of TCRαβ+ and CD4+ T cells, which depends on MHC function, is reduced in NUP96+/- mice. Upon immunization, Nup96+/- mice showed impaired antigen presentation and T cell proliferation. Nup96+/- cells and mice were highly susceptible to viral infection, demonstrating a role for Nup96 in innate and adaptive immunity.

UR - http://www.scopus.com/inward/record.url?scp=33644988814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644988814&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2006.01.014

DO - 10.1016/j.immuni.2006.01.014

M3 - Article

VL - 24

SP - 295

EP - 304

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 3

ER -