The NRAMPI, VDR and TNF-α Gene polymorphisms in Iranian tuberculosis patients: The study on host susceptibility

Muayad Merza, Parissa Farnia, Sabar Anoosheh, Mohammed Varahram, Mehdi Kazampour, Omid Pajand, Shima Saeif, Mehdi Mirsaeidi, Mohammad Reza Masjedi, Ali Akbar Velayati, Sven Hoffner

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The natural resistance-associated macrophage protein (NRAMP1), Vitamin-D receptor (VDR) and Tumor necrosis factor (TNF-α) have been associated in susceptibility to tuberculosis, but the results have been inconsistent. This study aimed to determine the association of NRAMP1, VDR, and TNF-á variant with development of pulmonary tuberculosis (PTB) among Iranian patients. The single nucleotide polymorphisms (SNPs) at INT4, D543, 3'UTR of NRAMP1 gene, SNPs in restriction sites of BsmI, and FokI of the VDR gene and SNPs of TNF-α at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among two groups of individual; patients with PTB (n=117) and healthy controls (n=60). Thereafter, the frequencies of extended haplotypes and diplotypes were estimated. No statistically significant differences were observed in allele frequencies of INT4, D543, 3'UTR of NRAMPI, FokI of VDR and TNF-α at -238, -244,-863 and -857 position. Although, the frequency of b allele of BsmI [ORs: 0.24 CI95% ( 0.07-0.67 (p=0.001)] and -308 A variant in TNF-α promoter region [ORs:0.26 CI95%( 0.07-0.77) (p=0.006)] were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes of NRAMP [GG TGTG++GA; 0.02(0.001-0.0035)], VDR [FFBB; 0.2(0.6-0.6] and TNF-α [CCCCGGGGGG; 0.49(0.25-0.97)] were statistically different in patients and control subjects (p<0.05). This study confirmed the association of SNPs in BsmI (B/b + b/b) of VDR and SNPs in -308A (G/A +G/G) of TNF-α genes with susceptibility to tuberculosis in Iranian PTB patients. Furthermore, the extended haplotypes and diplotypes analysis can be considered as an alternative way to determine the host susceptibility to TB.

Original languageEnglish (US)
Pages (from-to)252-256
Number of pages5
JournalBrazilian Journal of Infectious Diseases
Volume13
Issue number4
DOIs
StatePublished - Aug 2009

Keywords

  • NRAMP
  • Tnf-α gene
  • Tuberculosis
  • VDR

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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    Merza, M., Farnia, P., Anoosheh, S., Varahram, M., Kazampour, M., Pajand, O., Saeif, S., Mirsaeidi, M., Masjedi, M. R., Velayati, A. A., & Hoffner, S. (2009). The NRAMPI, VDR and TNF-α Gene polymorphisms in Iranian tuberculosis patients: The study on host susceptibility. Brazilian Journal of Infectious Diseases, 13(4), 252-256. https://doi.org/10.1590/S1413-86702009000400002