The NMDA and AMPA/KA receptors are involved in glutamate-induced alterations of occludin expression and phosphorylation in brain endothelial cells

Ibolya Edit Andras, Mária A. Deli, Szilvia Veszelka, Kentaro Hayashi, Bernhard Hennig, Michal J Toborek

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Glutamate levels increase dramatically in cerebral ischemia and stroke. This may lead to opening of the blood-brain barrier (BBB) and induce further brain damage. Because endothelial tight junctions are critical elements of the BBB integrity, the aim of this study was to investigate the mechanisms of glutamate-induced alterations of the tight-junction protein occludin in cultured brain microvascular endothelial cells (BMECs). Transient exposure to glutamate resulted in cellular redistribution of occludin, followed by a decrease in the total level of this protein and diminished barrier function of BMECs. Inhibition of the N-methyl-D-aspartate (NMDA) or alpha-amino-3-hydroxy-5-methylisoxazole- 4-propionate/kainate (AMPA/KA) receptors attenuated glutamate-induced changes in occludin redistribution but not in the total protein levels. Treatment with glutamate also increased tyrosine phosphorylation and decreased threonine phosphorylation of occludin. Inhibition of the NMDA receptors by MK-801 partially protected against glutamate-induced elevation of occludin tyrosine phosphorylation. In addition, pretreatment with MK-801-attenuated glutamate-mediated disruption of endothelial barrier function. Blocking of the AMPA/KA receptors by 6,7-dinitroquinoxaline-2.3-dione (DNQX) protected against hypophosphorylation of threonine residues of occludin; however, it did not affect disruption of endothelial integrity. These findings indicate the opposite effects of the NMDA and AMPA/KA receptors on occludin phosphorylation and disruption of the BBB functions.

Original languageEnglish
Pages (from-to)1431-1443
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue number8
DOIs
StatePublished - Aug 24 2007
Externally publishedYes

Fingerprint

Occludin
Kainic Acid Receptors
Propionates
N-Methylaspartate
Glutamic Acid
Endothelial Cells
Phosphorylation
Brain
Blood-Brain Barrier
Dizocilpine Maleate
Threonine
Tyrosine
Tight Junction Proteins
Tight Junctions
bucide
N-Methyl-D-Aspartate Receptors
Brain Ischemia
Proteins
Stroke

Keywords

  • Blood-brain barrier
  • Glutamate receptors
  • Occludin
  • Phosphorylation
  • Tight junction

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

The NMDA and AMPA/KA receptors are involved in glutamate-induced alterations of occludin expression and phosphorylation in brain endothelial cells. / Andras, Ibolya Edit; Deli, Mária A.; Veszelka, Szilvia; Hayashi, Kentaro; Hennig, Bernhard; Toborek, Michal J.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 27, No. 8, 24.08.2007, p. 1431-1443.

Research output: Contribution to journalArticle

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