The nitroreduction of chloramphenicol by human liver tissue

Z. Salem, T. Murray, Adel A Yunis

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Fresh-frozen human liver tissue was assayed for its ability to reduce the nitrogroup of RNO2 to the amine. All 10 livers examined exhibited demonstrable reductase activity. The reduction was potentiated by NADPH and abolished by boiling the liver homogenates. The nitroreductase activity varied among the different livers by as much as severalfold. These findings show the ability of the human liver to reduce RNO2 and support the hypothesis that certain toxic intermediates of the nitroreduction of RNO2 may be responsible for the aplastic anemia associated with this drug.

Original languageEnglish (US)
Pages (from-to)881-886
Number of pages6
JournalThe Journal of laboratory and clinical medicine
Volume97
Issue number6
StatePublished - 1981

Fingerprint

Chloramphenicol
Liver
Tissue
Nitroreductases
Aplastic Anemia
Poisons
NADP
Boiling liquids
Amines
Oxidoreductases
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The nitroreduction of chloramphenicol by human liver tissue. / Salem, Z.; Murray, T.; Yunis, Adel A.

In: The Journal of laboratory and clinical medicine, Vol. 97, No. 6, 1981, p. 881-886.

Research output: Contribution to journalArticle

Salem, Z, Murray, T & Yunis, AA 1981, 'The nitroreduction of chloramphenicol by human liver tissue', The Journal of laboratory and clinical medicine, vol. 97, no. 6, pp. 881-886.
Salem, Z. ; Murray, T. ; Yunis, Adel A. / The nitroreduction of chloramphenicol by human liver tissue. In: The Journal of laboratory and clinical medicine. 1981 ; Vol. 97, No. 6. pp. 881-886.
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