The nature of surface immunoglobulin in pure monocytic (M5) leukemia

R. R. Tubbs, R. Valenzuela, R. A. Savage, S. D. Deodhar

Research output: Contribution to journalArticle

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Abstract

The presence of endogenous monoclonal surface immunoglobulin (SIg) on circulating neoplastic cells of certain B-cells disorders such as the leukemic phase of lymphoma and B-cell acute lymphocytic leukemia is well established. The paradoxical appearance of prominent SIg on blasts from adults with acute nonlymphocytic leukemia has also been observed. In some cases, myeloblasts and myelomonoblasts have been characterized by polyclonal SIg of exogenous origin. Neoplastic cells from two patients with pure monocytic leukemia, a rare variant of acute nonlymphocytic leukemia, were studied with respect to functional properties. All blasts from both patients demonstrated polyclonal SIg. This surface fluorescence was not artifactual, since identical results were obtained when cells were stained with F(ab')2 fragments and whole test antisera. Trypsin digestion removed the immunoglobulin, and it was not reconstituted on the cell surface after short-term culture. The SIg observed was apparently of exogenous origin, bound to the Fc or cytophilic antibody receptor. Thus, SIg on leukemic blasts does not necessarily imply B-lymphocyte cytogenesis. The use of trypsin digestion to distinguish between exogenous and endogenous SIg may be useful in the subclassification of acute nonlymphocytic leukemia.

Original languageEnglish
Pages (from-to)614-617
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume72
Issue number4
StatePublished - Dec 1 1979
Externally publishedYes

Fingerprint

B-Cell Antigen Receptors
Leukemia
Acute Myeloid Leukemia
Trypsin
Digestion
B-Lymphocytes
B-Cell Leukemia
Circulating Neoplastic Cells
Granulocyte Precursor Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Immunoglobulins
Immune Sera
Lymphoma
Fluorescence
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Tubbs, R. R., Valenzuela, R., Savage, R. A., & Deodhar, S. D. (1979). The nature of surface immunoglobulin in pure monocytic (M5) leukemia. American Journal of Clinical Pathology, 72(4), 614-617.

The nature of surface immunoglobulin in pure monocytic (M5) leukemia. / Tubbs, R. R.; Valenzuela, R.; Savage, R. A.; Deodhar, S. D.

In: American Journal of Clinical Pathology, Vol. 72, No. 4, 01.12.1979, p. 614-617.

Research output: Contribution to journalArticle

Tubbs, RR, Valenzuela, R, Savage, RA & Deodhar, SD 1979, 'The nature of surface immunoglobulin in pure monocytic (M5) leukemia', American Journal of Clinical Pathology, vol. 72, no. 4, pp. 614-617.
Tubbs RR, Valenzuela R, Savage RA, Deodhar SD. The nature of surface immunoglobulin in pure monocytic (M5) leukemia. American Journal of Clinical Pathology. 1979 Dec 1;72(4):614-617.
Tubbs, R. R. ; Valenzuela, R. ; Savage, R. A. ; Deodhar, S. D. / The nature of surface immunoglobulin in pure monocytic (M5) leukemia. In: American Journal of Clinical Pathology. 1979 ; Vol. 72, No. 4. pp. 614-617.
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