The NAD-glycohydrolase (nga) gene of Streptococcus pyogenes

Dragana Ajdic, William M. McShan, Dragutin J. Savic, Dieter Gerlach, Joseph J. Ferretti

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The gene for NAD-glycohydrolase (nga) of group A streptococci (Streptococcus pyogenes) was identified and shown to be located immediately adjacent to the gene for streptolysin O (slo). The nga gene contains 1341 base pairs and encodes a protein of 447 amino acids, including an N-terminal signal peptide. Results from analysis with the polymerase chain reaction indicated that the nga gene is present in all of the strains tested. Functional extracellular NAD-glycohydrolase, also known as NADase, was detected among a wide variety of clinical isolates and known laboratory strains and shown to be present in 72% of 100 strains examined. In contrast, 92% of strains isolated from patients with invasive streptococcal infections were positive for NADase production. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalFEMS Microbiology Letters
Volume191
Issue number2
DOIs
StatePublished - Oct 15 2000
Externally publishedYes

Fingerprint

NAD+ Nucleosidase
Streptococcus pyogenes
Genes
Streptococcal Infections
Protein Sorting Signals
Streptococcus
Base Pairing
Amino Acids
Polymerase Chain Reaction

Keywords

  • NAD-glycohydrolase
  • NADase
  • Streptococcus pyogenes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The NAD-glycohydrolase (nga) gene of Streptococcus pyogenes. / Ajdic, Dragana; McShan, William M.; Savic, Dragutin J.; Gerlach, Dieter; Ferretti, Joseph J.

In: FEMS Microbiology Letters, Vol. 191, No. 2, 15.10.2000, p. 235-241.

Research output: Contribution to journalArticle

Ajdic, D, McShan, WM, Savic, DJ, Gerlach, D & Ferretti, JJ 2000, 'The NAD-glycohydrolase (nga) gene of Streptococcus pyogenes', FEMS Microbiology Letters, vol. 191, no. 2, pp. 235-241. https://doi.org/10.1016/S0378-1097(00)00396-7
Ajdic, Dragana ; McShan, William M. ; Savic, Dragutin J. ; Gerlach, Dieter ; Ferretti, Joseph J. / The NAD-glycohydrolase (nga) gene of Streptococcus pyogenes. In: FEMS Microbiology Letters. 2000 ; Vol. 191, No. 2. pp. 235-241.
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