The molecular effects of skeletal muscle myosin regulatory light chain phosphorylation

Michael J. Greenberg, Tanya R. Mealy, James D. Watt, Michelle Jones, Danuta Szczesna-Cordary, Jeffrey R. Moore

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Phosphorylation of the myosin regulatory light chain (RLC) in skeletal muscle has been proposed to act as a molecular memory of recent activation by increasing the rate of force development, ATPase activity, and isometric force at submaximal activation in fibers. It has been proposed that these effects stem from phosphorylation-induced movement of myosin heads away from the thick filament backbone. In this study, we examined the molecular effects of skeletal muscle myosin RLC phosphorylation using in vitro motility assays. We showed that, independently of the thick filament backbone, the velocity of skeletal muscle myosin is decreased upon phosphorylation due to an increase in the myosin duty cycle. Furthermore, we did not observe a phosphorylation-dependent shift in calcium sensitivity in the absence of the myosin thick filament. These data suggest that phosphorylation-induced movement of myosin heads away from the thick filament backbone explains only part of the observed phosphorylation- induced changes in myosin mechanics. Last, we showed that the duty cycle of skeletal muscle myosin is strain dependent, consistent with the notion that strain slows the rate of ADP release in striated muscle.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume297
Issue number2
DOIs
StatePublished - Aug 1 2009

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Skeletal Muscle Myosins
Myosin Light Chains
Myosins
Phosphorylation
Head Movements
Striated Muscle
Mechanics
Adenosine Diphosphate
Adenosine Triphosphatases
Skeletal Muscle
Calcium

Keywords

  • In vitro motility assay
  • Mechanics

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

The molecular effects of skeletal muscle myosin regulatory light chain phosphorylation. / Greenberg, Michael J.; Mealy, Tanya R.; Watt, James D.; Jones, Michelle; Szczesna-Cordary, Danuta; Moore, Jeffrey R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 297, No. 2, 01.08.2009.

Research output: Contribution to journalArticle

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AU - Moore, Jeffrey R.

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