The MHC E locus in macaques is polymorphic and is conserved between macaques and humans

Jonathan E. Boyson, Stephen N. McAdam, Awen Gallimore, Thaddeus G. Golos, Xiaomin Liu, Frances M. Gotch, Austin L. Hughes, David Watkins

Research output: Contribution to journalArticle

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Abstract

Although the functions of the molecules encoded by the classical MHC class I loci are well defined, no function has been ascribed to the molecules encoded by the non-classical MHC class I loci. To investigate the evolution and conservation of the non-classical loci, we cloned and sequenced HLA-E homologues in macaques. We isolated four E locus alleles from five rhesus monkeys and two E locus alleles from one cynomolgus monkey, which indicated that the E locus in macaques is polymorphic. We also compared the rate of nucleotide substitution in the second intron of the macaque and human E locus alleles with that of exons two and three. The rate of nucleotide substitution was significantly higher in the introns, which suggested that the E locus has evolved under selective pressure. Additionally, comparison of the rates of synonymous and non-synonymous substitutions in the peptide binding region versus the remainder of the molecule suggested that the codons encoding the amino acids in the peptide binding region had been conserved in macaques and humans over the 36 million years since macaques and humans last shared a common ancestor.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalImmunogenetics
Volume41
Issue number2-3
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Macaca
Alleles
Introns
Nucleotides
Peptides
Macaca fascicularis
Macaca mulatta
Codon
Exons
Amino Acids

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Boyson, J. E., McAdam, S. N., Gallimore, A., Golos, T. G., Liu, X., Gotch, F. M., ... Watkins, D. (1995). The MHC E locus in macaques is polymorphic and is conserved between macaques and humans. Immunogenetics, 41(2-3), 59-68. https://doi.org/10.1007/BF00182314

The MHC E locus in macaques is polymorphic and is conserved between macaques and humans. / Boyson, Jonathan E.; McAdam, Stephen N.; Gallimore, Awen; Golos, Thaddeus G.; Liu, Xiaomin; Gotch, Frances M.; Hughes, Austin L.; Watkins, David.

In: Immunogenetics, Vol. 41, No. 2-3, 01.01.1995, p. 59-68.

Research output: Contribution to journalArticle

Boyson, JE, McAdam, SN, Gallimore, A, Golos, TG, Liu, X, Gotch, FM, Hughes, AL & Watkins, D 1995, 'The MHC E locus in macaques is polymorphic and is conserved between macaques and humans', Immunogenetics, vol. 41, no. 2-3, pp. 59-68. https://doi.org/10.1007/BF00182314
Boyson JE, McAdam SN, Gallimore A, Golos TG, Liu X, Gotch FM et al. The MHC E locus in macaques is polymorphic and is conserved between macaques and humans. Immunogenetics. 1995 Jan 1;41(2-3):59-68. https://doi.org/10.1007/BF00182314
Boyson, Jonathan E. ; McAdam, Stephen N. ; Gallimore, Awen ; Golos, Thaddeus G. ; Liu, Xiaomin ; Gotch, Frances M. ; Hughes, Austin L. ; Watkins, David. / The MHC E locus in macaques is polymorphic and is conserved between macaques and humans. In: Immunogenetics. 1995 ; Vol. 41, No. 2-3. pp. 59-68.
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