The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization

J. Tschopp, E. R. Podack, H. J. Muller-Eberhard

Research output: Contribution to journalArticle

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Abstract

Polymerization of C9 occurs spontaneously or can be induced by the tetramolecular complex C5b-8. Spontaneous C9 (0.15 mg/ml) polymerization required more than 3 days at 37° C. In the presence of C5b-8, C9 polymerization was complete within 10 min. The molar C9:C5b-8 ratio determined the extent of tubular poly C9 formation by C5b-8-bearing phospholipid vesicles. When this ratio was 9:1 or 12:1, 72% of complex-bound C9 was present as SDS resistant tubular poly C9 (M(r) = 1.1 x 106). At lower C9:C5b-8 ratios, poly C9 was bound primarily in nontubular form. Tubular poly C9, as part of C5b-9, could also be generated on rabbit erythrocytes by using whole human serum as a complement source. At limiting serum concentration (molar C9 to C8 ratio approximately 2), no SDS-resistant tubular poly C9 was detected. At high serum concentration or when using serum that was supplemented with C9, up to 40% of the C9 was SDS-resistant tubular poly C9, and the rest was poly C9, which was incompletely polymerized. It is suggested that the C5b-8 complex acts as an accelerator of C9 polymerization, and that its relative concentration to C9 determines the ultrastructure of the C5b-9 complex.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalJournal of Immunology
Volume134
Issue number1
StatePublished - Jan 1 1985
Externally publishedYes

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Complement Membrane Attack Complex
Polymerization
Serum
Phospholipids
Erythrocytes
complement C9 polymer
complement C5b-8 complex
Rabbits

ASJC Scopus subject areas

  • Immunology

Cite this

Tschopp, J., Podack, E. R., & Muller-Eberhard, H. J. (1985). The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization. Journal of Immunology, 134(1), 495-499.

The membrane attack complex of complement : C5b-8 complex as accelerator of C9 polymerization. / Tschopp, J.; Podack, E. R.; Muller-Eberhard, H. J.

In: Journal of Immunology, Vol. 134, No. 1, 01.01.1985, p. 495-499.

Research output: Contribution to journalArticle

Tschopp, J, Podack, ER & Muller-Eberhard, HJ 1985, 'The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization', Journal of Immunology, vol. 134, no. 1, pp. 495-499.
Tschopp, J. ; Podack, E. R. ; Muller-Eberhard, H. J. / The membrane attack complex of complement : C5b-8 complex as accelerator of C9 polymerization. In: Journal of Immunology. 1985 ; Vol. 134, No. 1. pp. 495-499.
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