The membrane attack complex of complement and its precursor proteins lack phospholipase activity

Carl Wilhelm Vogel, Andreas Plückthun, Eckhard R. Podack, Edward A. Dennis, Hans J. Müller-Eberhardb

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The membrane attack complex of human complement and its highly purified precursor proteins have been analyzed for phospholipase activity. Using three different sensitive assays, phospholipase a1, A2, C or D activity could not be detected. Based on the sensitivity of the assays employed, these results indicate the complement-mediated membrane damage is not enhanced by covalent breakdown of membrane phospholipids, but is entirely caused by physical action of the membrane attack complex. The results also imply that the putative serine esterase sites of C6 and C7 are not acting on phospholipids.

Original languageEnglish (US)
Pages (from-to)377-382
Number of pages6
JournalMolecular Immunology
Volume20
Issue number4
DOIs
StatePublished - Apr 1983

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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