Abstract
Juvenile rainbow trout were exposed to 0, 0.1, 1, 3, and 5 μg/l silver (Ag, as AgNO3) for 23 days. Specific growth rate, cumulative food consumption, food-conversion efficiency, and critical swimming speed (Ucrit) were significantly reduced during 5 μg/l Ag exposure, demonstrating a physiological cost of silver acclimation. Only the 5 μg/l Ag treatment had significant cumulative mortality (5.2%). Fish were most susceptible to silver on days 5 and 15. Exposure to 5 μg/l Ag significantly lowered plasma Na+ and Cl- on days 5 and 10, but plasma ion concentration recovered thereafter. Unidirectional Na+ uptake and gill Na/K-ATPase activity were significantly inhibited by 3 and 5 μg/l Ag exposure. Na+ uptake was inhibited by 3 μg/l Ag at day 5 alone, whereas the effects at the highest Ag exposure persisted until day 15. Gill Na/K-ATPase was inhibited on day 5 in both the 3 and 5 μg/l Ag treatments but increased to approx. 1.5 times of control levels by day 23. Only the 3 and 5 μg/l Ag treatments produced toxicological acclimation (at least twofold elevations in 168-h LC50 values in fish subsampled on days 15 and 23). We conclude that physiological acclimation results from compensatory changes in Na+ transport at the gills, and that these changes may eventually lead to toxicological acclimation.
| Original language | English |
|---|---|
| Pages (from-to) | 587-597 |
| Number of pages | 11 |
| Journal | Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology |
| Volume | 172 |
| Issue number | 7 |
| DOIs | |
| State | Published - Dec 1 2002 |
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Keywords
- Metabolic costs
- Na/K-ATPase
- Rainbow trout
- Silver acclimation
- Unidirectional influx
ASJC Scopus subject areas
- Animal Science and Zoology
- Environmental Science(all)
- Physiology
- Physiology (medical)
Cite this
The mechanisms and costs of physiological and toxicological acclimation to waterborne silver in juvenile rainbow trout (Oncorhynchus mykiss). / Galvez, F.; Wood, C. M.
In: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, Vol. 172, No. 7, 01.12.2002, p. 587-597.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The mechanisms and costs of physiological and toxicological acclimation to waterborne silver in juvenile rainbow trout (Oncorhynchus mykiss)
AU - Galvez, F.
AU - Wood, C. M.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Juvenile rainbow trout were exposed to 0, 0.1, 1, 3, and 5 μg/l silver (Ag, as AgNO3) for 23 days. Specific growth rate, cumulative food consumption, food-conversion efficiency, and critical swimming speed (Ucrit) were significantly reduced during 5 μg/l Ag exposure, demonstrating a physiological cost of silver acclimation. Only the 5 μg/l Ag treatment had significant cumulative mortality (5.2%). Fish were most susceptible to silver on days 5 and 15. Exposure to 5 μg/l Ag significantly lowered plasma Na+ and Cl- on days 5 and 10, but plasma ion concentration recovered thereafter. Unidirectional Na+ uptake and gill Na/K-ATPase activity were significantly inhibited by 3 and 5 μg/l Ag exposure. Na+ uptake was inhibited by 3 μg/l Ag at day 5 alone, whereas the effects at the highest Ag exposure persisted until day 15. Gill Na/K-ATPase was inhibited on day 5 in both the 3 and 5 μg/l Ag treatments but increased to approx. 1.5 times of control levels by day 23. Only the 3 and 5 μg/l Ag treatments produced toxicological acclimation (at least twofold elevations in 168-h LC50 values in fish subsampled on days 15 and 23). We conclude that physiological acclimation results from compensatory changes in Na+ transport at the gills, and that these changes may eventually lead to toxicological acclimation.
AB - Juvenile rainbow trout were exposed to 0, 0.1, 1, 3, and 5 μg/l silver (Ag, as AgNO3) for 23 days. Specific growth rate, cumulative food consumption, food-conversion efficiency, and critical swimming speed (Ucrit) were significantly reduced during 5 μg/l Ag exposure, demonstrating a physiological cost of silver acclimation. Only the 5 μg/l Ag treatment had significant cumulative mortality (5.2%). Fish were most susceptible to silver on days 5 and 15. Exposure to 5 μg/l Ag significantly lowered plasma Na+ and Cl- on days 5 and 10, but plasma ion concentration recovered thereafter. Unidirectional Na+ uptake and gill Na/K-ATPase activity were significantly inhibited by 3 and 5 μg/l Ag exposure. Na+ uptake was inhibited by 3 μg/l Ag at day 5 alone, whereas the effects at the highest Ag exposure persisted until day 15. Gill Na/K-ATPase was inhibited on day 5 in both the 3 and 5 μg/l Ag treatments but increased to approx. 1.5 times of control levels by day 23. Only the 3 and 5 μg/l Ag treatments produced toxicological acclimation (at least twofold elevations in 168-h LC50 values in fish subsampled on days 15 and 23). We conclude that physiological acclimation results from compensatory changes in Na+ transport at the gills, and that these changes may eventually lead to toxicological acclimation.
KW - Metabolic costs
KW - Na/K-ATPase
KW - Rainbow trout
KW - Silver acclimation
KW - Unidirectional influx
UR - http://www.scopus.com/inward/record.url?scp=0036931736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036931736&partnerID=8YFLogxK
U2 - 10.1007/s00360-002-0287-7
DO - 10.1007/s00360-002-0287-7
M3 - Article
C2 - 12355227
AN - SCOPUS:0036931736
VL - 172
SP - 587
EP - 597
JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
SN - 0174-1578
IS - 7
ER -