The maternal-zygotic transition and zygotic activation of the Mnemiopsis leidyi genome occurs within the first three cleavage cycles

Phillip L. Davidson, Bernard J. Koch, Christine E. Schnitzler, Jonathan Q. Henry, Mark Q. Martindale, Andreas D. Baxevanis, William Browne

Research output: Contribution to journalArticle

Abstract

The maternal-zygotic transition (MZT) describes the developmental reprogramming of gene expression marked by the degradation of maternally supplied gene products and activation of the zygotic genome. While the timing and duration of the MZT vary among taxa, little is known about early-stage transcriptional dynamics in the non-bilaterian phylum Ctenophora. We sought to better understand the extent of maternal mRNA loading and subsequent differential transcript abundance during the earliest stages of development by performing comprehensive RNA-sequencing-based analyses of mRNA abundance in single- and eight-cell stage embryos in the lobate ctenophore Mnemiopsis leidyi. We found 1,908 contigs with significant differential abundance between single- and eight-cell stages, of which 1,208 contigs were more abundant at the single-cell stage and 700 contigs were more abundant at the eight-cell stage. Of the differentially abundant contigs, 267 were exclusively present in the eight-cell samples, providing strong evidence that both the MZT and zygotic genome activation (ZGA) have commenced by the eight-cell stage. Many highly abundant transcripts encode genes involved in molecular mechanisms critical to the MZT, such as maternal transcript degradation, serine/threonine kinase activity, and chromatin remodeling. Our results suggest that chromosomal restructuring, which is critical to ZGA and the initiation of transcriptional regulation necessary for normal development, begins by the third cleavage within 1.5 hr post-fertilization in M. leidyi.

Original languageEnglish (US)
Pages (from-to)1218-1229
Number of pages12
JournalMolecular Reproduction and Development
Volume84
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Ctenophora
Mothers
Genome
Transcriptional Activation
Stored Messenger RNA
RNA Sequence Analysis
Developmental Genes
Chromatin Assembly and Disassembly
Protein-Serine-Threonine Kinases
Fertilization
Embryonic Structures
Gene Expression
Messenger RNA

Keywords

  • chromatin remodeling
  • ctenophora
  • gene expression
  • maternal transcript degradation
  • RNAseq

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

The maternal-zygotic transition and zygotic activation of the Mnemiopsis leidyi genome occurs within the first three cleavage cycles. / Davidson, Phillip L.; Koch, Bernard J.; Schnitzler, Christine E.; Henry, Jonathan Q.; Martindale, Mark Q.; Baxevanis, Andreas D.; Browne, William.

In: Molecular Reproduction and Development, Vol. 84, No. 11, 01.11.2017, p. 1218-1229.

Research output: Contribution to journalArticle

Davidson, Phillip L. ; Koch, Bernard J. ; Schnitzler, Christine E. ; Henry, Jonathan Q. ; Martindale, Mark Q. ; Baxevanis, Andreas D. ; Browne, William. / The maternal-zygotic transition and zygotic activation of the Mnemiopsis leidyi genome occurs within the first three cleavage cycles. In: Molecular Reproduction and Development. 2017 ; Vol. 84, No. 11. pp. 1218-1229.
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