Abstract
To better characterize the proposed growth modulatory functions of mast cells and their products in the regulation of epidermal homeostasis, we have assessed keratinocyte proliferation by incorporation of [3H]thymidine in murine skin organ cultures after incubation with different concentrations of histamine, serotonin or TNF-alpha. Both histamine and serotonin significantly stimulate the proliferation of keratinocytes as compared to controls (P < 0.001), reaching maxima within a range from micro- to nanomolar concentrations. Even at subnanomolar concentration, TNF-alpha significantly inhibits the incorporation of labelled thyroidine by epidermal keratinocytes in murine skin organ culture (P < 0.001). These observations support the concept of mast cell products as modulators of keratinocyte proliferation, and suggest a role for mast cells in the regulation of epidermal tissue turnover under physiological conditions.
Original language | English (US) |
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Pages (from-to) | 79-84 |
Number of pages | 6 |
Journal | Journal of dermatological science |
Volume | 16 |
Issue number | 1 |
DOIs | |
State | Published - Nov 1997 |
Externally published | Yes |
Keywords
- Histamine
- Keratinocyte
- Mast cell
- Mouse
- Serotonin
- TNF-alpha
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology