The long non-coding RNA FMR4 promotes proliferation of human neural precursor cells and epigenetic regulation of gene expression in trans

Veronica J. Peschansky, Chiara Pastori, Zane R Zeier, Katya Wentzel, Dmitry Velmeshev, Marco Magistri, José P. Silva, Claes R Wahlestedt

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Triplet repeat expansions in the Fragile X mental retardation 1 (FMR1) gene cause either intellectual disability and autism, or adult-onset neurodegeneration, with poorly understood variability in presentation. Previous studies have identified several long noncoding RNAs (lncRNAs) at the FMR1 locus, including FMR4. Similarly to FMR1, FMR4 is silenced by large-repeat expansions that result in enrichment of DNA and histone methylation within the shared promoter and repeat sequence, suggesting a possible role for this noncoding RNA in the pathophysiology of Fragile X. We therefore assessed the functional role of FMR4 to gain further insight into the molecular processes in Fragile X-associated disorders. Previous work showed that FMR4 does not exhibit cis-regulation of FMR1. Here, we found that FMR4 is a chromatin-associated transcript and, using genome-wide chromatin immunoprecipitation experiments, showed that FMR4 alters the chromatin state and the expression of several hundred genes in trans. Among the genes regulated by FMR4, we found enrichment for those involved in neural development and cellular proliferation. S-phase marker assays further demonstrated that FMR4 may promote cellular proliferation, rather than differentiation, of human neural precursor cells (hNPCs).By establishing this novel function for FMR4 in hNPCs, we lend support to existing evidence of the epigenetic involvement of lncRNA in nervous system development, and increase our understanding of the complex pathogenesis underlying neurological disorders associated with FMR1 repeat expansions.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume74
DOIs
StatePublished - Jul 1 2016

Fingerprint

Long Noncoding RNA
Gene Expression Regulation
Epigenomics
Intellectual Disability
Chromatin
Cell Proliferation
Genes
Trinucleotide Repeats
Untranslated RNA
Chromatin Immunoprecipitation
DNA Methylation
Autistic Disorder
Nervous System Diseases
S Phase
Histones
Nervous System
Genome

Keywords

  • Cellular proliferation
  • Chromatin modification
  • Epigenetics
  • Fragile X
  • Intellectual disability
  • LncRNA
  • Neurodevelopment

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

The long non-coding RNA FMR4 promotes proliferation of human neural precursor cells and epigenetic regulation of gene expression in trans. / Peschansky, Veronica J.; Pastori, Chiara; Zeier, Zane R; Wentzel, Katya; Velmeshev, Dmitry; Magistri, Marco; Silva, José P.; Wahlestedt, Claes R.

In: Molecular and Cellular Neuroscience, Vol. 74, 01.07.2016, p. 49-57.

Research output: Contribution to journalArticle

Peschansky, Veronica J. ; Pastori, Chiara ; Zeier, Zane R ; Wentzel, Katya ; Velmeshev, Dmitry ; Magistri, Marco ; Silva, José P. ; Wahlestedt, Claes R. / The long non-coding RNA FMR4 promotes proliferation of human neural precursor cells and epigenetic regulation of gene expression in trans. In: Molecular and Cellular Neuroscience. 2016 ; Vol. 74. pp. 49-57.
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