The limited capacity of malignant glioma-derived exosomes to suppress peripheral immune effectors

J. Bryan Iorgulescu, Michael E. Ivan, Michael Safaee, Andrew T. Parsa

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Tumor-derived microvesicular exosomes permit intercellular communication both locally and systemically by delivering a snapshot of the tumor cell's constituents. We thus investigated whether exosomes mediate malignant glioma's facility for inducing peripheral immunosuppression. In Western blot and RT-PCR analyses, glioma-derived exosomes displayed exosome-specific markers, but failed to recapitulate the antigen-presentation machinery, surface co-modulatory signals, or immunosuppressive mediator status of their parent tumor cells. Treatment with glioma-derived exosomes promoted immunosuppressive HLA-DRlow monocytic phenotypes, but failed to induce monocytic PD-L1 expression or alter the activation of cytotoxic T-cells from patients' peripheral blood by FACS and RT-PCR analyses. Our results suggest that malignant glioma-derived exosomes are restricted in their capacity to directly prime peripheral immunosuppression.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalJournal of Neuroimmunology
Volume290
DOIs
StatePublished - Jan 15 2016

Keywords

  • Exosome
  • Glioma
  • Immunosuppression
  • Immunotherapy
  • Microvesicle

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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