The levels of integrin α(v)β5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells

K. Takayama, H. Ueno, Xin-Hai Pei, Y. Nakanishi, J. Yatsunami, N. Hara

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Adenovirus-mediated transfer of growth-inhibiting molecules, such as p53, shows promise as an effective method of suppressing the growth of cancer cells. As the basis for in vivo studies, we examined transfection efficiency using 15 human lung cancer cell lines that differ in their endogenous p53 status. When infected with an adenovirus expressing bacterial β-galactosidase, the different cell lines showed different levels of β-galactosidase activity. We found a correlation between the level of integrin α(v)β5, which is thought to be an adherence receptor for adenoviruses, and the expression level of the transferred gene, suggesting that gene expression is largely dependent on the infection efficiency. Growth inhibition was induced in all cell lines tested following infection with an adenovirus containing p53, regardless of the genetic status of their endogenous p53 provided a sufficient amount of p53 protein was expressed. Our results (1) confirm that the examination of the susceptibility of target cancer cells to an adenovirus is important when considering performing adenovirus-mediated gene transfer and for evaluating its therapeutic effects; and (2) suggest that the quantification of integrin α(v)β5 may be a good way of predicting the susceptibility of cells to adenoviral vectors.

Original languageEnglish (US)
Pages (from-to)361-368
Number of pages8
JournalGene Therapy
Volume5
Issue number3
StatePublished - 1998
Externally publishedYes

Fingerprint

Adenoviridae
Integrins
Lung Neoplasms
Galactosidases
Cell Line
Genes
Growth
Adenoviridae Infections
Therapeutic Uses
Transfection
Neoplasms
Gene Expression
Infection
Proteins

Keywords

  • Adenovirus-mediated gene transfer
  • Integrin
  • Lung cancer cells
  • p53
  • Susceptibility

ASJC Scopus subject areas

  • Genetics

Cite this

The levels of integrin α(v)β5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells. / Takayama, K.; Ueno, H.; Pei, Xin-Hai; Nakanishi, Y.; Yatsunami, J.; Hara, N.

In: Gene Therapy, Vol. 5, No. 3, 1998, p. 361-368.

Research output: Contribution to journalArticle

Takayama, K. ; Ueno, H. ; Pei, Xin-Hai ; Nakanishi, Y. ; Yatsunami, J. ; Hara, N. / The levels of integrin α(v)β5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells. In: Gene Therapy. 1998 ; Vol. 5, No. 3. pp. 361-368.
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