The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation

Lan Wang, Alexander Gural, Xiao Jian Sun, Xinyang Zhao, Fabiana Perna, Gang Huang, Megan A. Hatlen, Ly Vu, Fan Liu, Haiming Xu, Takashi Asai, Hao Xu, Tony Deblasio, Silvia Menendez, Francesca Voza, Yanwen Jiang, Philip A. Cole, Jinsong Zhang, Ari Melnick, Robert G. RoederStephen D Nimer

Research output: Contribution to journalArticle

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Abstract

The chromosomal translocations found in acute myelogenous leukemia (AML) generate oncogenic fusion transcription factors with aberrant transcriptional regulatory properties. Although therapeutic targeting of most leukemia fusion proteins remains elusive, the posttranslational modifications that control their function could be targetable. We found that AML1-ETO, the fusion protein generated by the t(8;21) translocation, is acetylated by the transcriptional coactivator p300 in leukemia cells isolated from t(8;21) AML patients, and that this acetylation is essential for its self-renewal-promoting effects in human cord blood CD34+ cells and its leukemogenicity in mouse models. Inhibition of p300 abrogates the acetylation of AML1-ETO and impairs its ability to promote leukemic transformation. Thus, lysine acetyltransferases represent a potential therapeutic target in AML.

Original languageEnglish
Pages (from-to)765-769
Number of pages5
JournalScience
Volume333
Issue number6043
DOIs
StatePublished - Aug 5 2011
Externally publishedYes

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Acetylation
Acute Myeloid Leukemia
Lysine
Leukemia
Genetic Translocation
Post Translational Protein Processing
Fetal Blood
Blood Cells
Proteins
Transcription Factors
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Wang, L., Gural, A., Sun, X. J., Zhao, X., Perna, F., Huang, G., ... Nimer, S. D. (2011). The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. Science, 333(6043), 765-769. https://doi.org/10.1126/science.1201662

The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. / Wang, Lan; Gural, Alexander; Sun, Xiao Jian; Zhao, Xinyang; Perna, Fabiana; Huang, Gang; Hatlen, Megan A.; Vu, Ly; Liu, Fan; Xu, Haiming; Asai, Takashi; Xu, Hao; Deblasio, Tony; Menendez, Silvia; Voza, Francesca; Jiang, Yanwen; Cole, Philip A.; Zhang, Jinsong; Melnick, Ari; Roeder, Robert G.; Nimer, Stephen D.

In: Science, Vol. 333, No. 6043, 05.08.2011, p. 765-769.

Research output: Contribution to journalArticle

Wang, L, Gural, A, Sun, XJ, Zhao, X, Perna, F, Huang, G, Hatlen, MA, Vu, L, Liu, F, Xu, H, Asai, T, Xu, H, Deblasio, T, Menendez, S, Voza, F, Jiang, Y, Cole, PA, Zhang, J, Melnick, A, Roeder, RG & Nimer, SD 2011, 'The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation', Science, vol. 333, no. 6043, pp. 765-769. https://doi.org/10.1126/science.1201662
Wang L, Gural A, Sun XJ, Zhao X, Perna F, Huang G et al. The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. Science. 2011 Aug 5;333(6043):765-769. https://doi.org/10.1126/science.1201662
Wang, Lan ; Gural, Alexander ; Sun, Xiao Jian ; Zhao, Xinyang ; Perna, Fabiana ; Huang, Gang ; Hatlen, Megan A. ; Vu, Ly ; Liu, Fan ; Xu, Haiming ; Asai, Takashi ; Xu, Hao ; Deblasio, Tony ; Menendez, Silvia ; Voza, Francesca ; Jiang, Yanwen ; Cole, Philip A. ; Zhang, Jinsong ; Melnick, Ari ; Roeder, Robert G. ; Nimer, Stephen D. / The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. In: Science. 2011 ; Vol. 333, No. 6043. pp. 765-769.
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AU - Liu, Fan

AU - Xu, Haiming

AU - Asai, Takashi

AU - Xu, Hao

AU - Deblasio, Tony

AU - Menendez, Silvia

AU - Voza, Francesca

AU - Jiang, Yanwen

AU - Cole, Philip A.

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AU - Melnick, Ari

AU - Roeder, Robert G.

AU - Nimer, Stephen D

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AB - The chromosomal translocations found in acute myelogenous leukemia (AML) generate oncogenic fusion transcription factors with aberrant transcriptional regulatory properties. Although therapeutic targeting of most leukemia fusion proteins remains elusive, the posttranslational modifications that control their function could be targetable. We found that AML1-ETO, the fusion protein generated by the t(8;21) translocation, is acetylated by the transcriptional coactivator p300 in leukemia cells isolated from t(8;21) AML patients, and that this acetylation is essential for its self-renewal-promoting effects in human cord blood CD34+ cells and its leukemogenicity in mouse models. Inhibition of p300 abrogates the acetylation of AML1-ETO and impairs its ability to promote leukemic transformation. Thus, lysine acetyltransferases represent a potential therapeutic target in AML.

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